Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21963 | 3' | -55.2 | NC_004914.1 | + | 20857 | 0.66 | 0.75445 |
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Target: 5'- gCGCCCGGACaaccguuaUuucacggaugagaaaAGCGCCAugGGGAGuGa -3' miRNA: 3'- -GUGGGUCUG--------G---------------UCGCGGUugUUCUCuC- -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 38117 | 0.66 | 0.739883 |
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Target: 5'- -cCCCAGGCCAGUGCUuuAGCGuuaacuuccGGAGc- -3' miRNA: 3'- guGGGUCUGGUCGCGG--UUGU---------UCUCuc -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 62093 | 0.67 | 0.653495 |
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Target: 5'- uCAgCCAGGacguCCAGCGUCAGCAAuGAGc- -3' miRNA: 3'- -GUgGGUCU----GGUCGCGGUUGUU-CUCuc -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 8572 | 0.68 | 0.620474 |
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Target: 5'- aGgCCAGGCUGGCGCaggagAGCGccGGAGAGa -3' miRNA: 3'- gUgGGUCUGGUCGCGg----UUGU--UCUCUC- -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 45952 | 0.68 | 0.609474 |
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Target: 5'- cCAUCUuaGGGCgAGCGCCAaugACAaacAGAGAGc -3' miRNA: 3'- -GUGGG--UCUGgUCGCGGU---UGU---UCUCUC- -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 21147 | 0.69 | 0.565761 |
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Target: 5'- uGCCCGuGGCaacGCGCCGACGGcAGAGg -3' miRNA: 3'- gUGGGU-CUGgu-CGCGGUUGUUcUCUC- -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 22575 | 0.69 | 0.565761 |
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Target: 5'- --aCCGGACCAGaaCGCCG-CAAGGGGa -3' miRNA: 3'- gugGGUCUGGUC--GCGGUuGUUCUCUc -5' |
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| 21963 | 3' | -55.2 | NC_004914.1 | + | 16925 | 1.09 | 0.001052 |
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Target: 5'- gCACCCAGACCAGCGCCAACAAGAGAGg -3' miRNA: 3'- -GUGGGUCUGGUCGCGGUUGUUCUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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