miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21969 3' -52.9 NC_004914.1 + 12622 0.66 0.867769
Target:  5'- uGCAUAUgaGGcACCACCCGAUGuucgacgccgGGCGa -3'
miRNA:   3'- -UGUGUGggCUaUGGUGGGCUAU----------UCGU- -5'
21969 3' -52.9 NC_004914.1 + 58651 0.67 0.785806
Target:  5'- aGCACGCCUauUGCCGCCCacUGAGaCGg -3'
miRNA:   3'- -UGUGUGGGcuAUGGUGGGcuAUUC-GU- -5'
21969 3' -52.9 NC_004914.1 + 12052 0.69 0.668662
Target:  5'- aACACAUCCugcuUACCAUCCGGUuucguguaacAGGCAa -3'
miRNA:   3'- -UGUGUGGGcu--AUGGUGGGCUA----------UUCGU- -5'
21969 3' -52.9 NC_004914.1 + 21050 0.72 0.547004
Target:  5'- gUAUugCUGAUGCUgACCCGAUgGAGCAg -3'
miRNA:   3'- uGUGugGGCUAUGG-UGGGCUA-UUCGU- -5'
21969 3' -52.9 NC_004914.1 + 13405 0.72 0.547004
Target:  5'- uGC-CACCUGAUACCGCaaaCGgAUAGGCu -3'
miRNA:   3'- -UGuGUGGGCUAUGGUGg--GC-UAUUCGu -5'
21969 3' -52.9 NC_004914.1 + 54312 0.73 0.462925
Target:  5'- uGCAUgguGCCgGGUGCCuCCCGGUGAGUu -3'
miRNA:   3'- -UGUG---UGGgCUAUGGuGGGCUAUUCGu -5'
21969 3' -52.9 NC_004914.1 + 22230 1.09 0.002101
Target:  5'- uACACACCCGAUACCACCCGAUAAGCAg -3'
miRNA:   3'- -UGUGUGGGCUAUGGUGGGCUAUUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.