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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21970 | 3' | -60.4 | NC_004914.1 | + | 57053 | 0.66 | 0.521805 |
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Target: 5'- gCAGUG-CCCgUGCgCAgCACGACGa -3' miRNA: 3'- aGUCGCgGGGgACGaGUgGUGCUGCc -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 54717 | 0.66 | 0.492004 |
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Target: 5'- cUCAGCGCCUUCUGUUgCGCCA-GAa-- -3' miRNA: 3'- -AGUCGCGGGGGACGA-GUGGUgCUgcc -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 9206 | 0.66 | 0.472579 |
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Target: 5'- gCAGCGCUgCCUGCcggaccuggcUCuCCACGA-GGu -3' miRNA: 3'- aGUCGCGGgGGACG----------AGuGGUGCUgCC- -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 59256 | 0.69 | 0.349017 |
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Target: 5'- cCAGCGCgCUCUcCUgGUCACGACGGg -3' miRNA: 3'- aGUCGCGgGGGAcGAgUGGUGCUGCC- -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 26171 | 0.7 | 0.289477 |
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Target: 5'- gCGGCGUCUCUUGC-CAaaaaCAUGACGGu -3' miRNA: 3'- aGUCGCGGGGGACGaGUg---GUGCUGCC- -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 8912 | 0.7 | 0.282621 |
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Target: 5'- --cGCuCCCCCUGUucgcccuucUCACCACG-CGGa -3' miRNA: 3'- aguCGcGGGGGACG---------AGUGGUGCuGCC- -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 19447 | 0.73 | 0.175741 |
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Target: 5'- aUCAGC-CCCCCUGCU-GCCAcCGGCa- -3' miRNA: 3'- -AGUCGcGGGGGACGAgUGGU-GCUGcc -5' |
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| 21970 | 3' | -60.4 | NC_004914.1 | + | 26297 | 1.1 | 0.000366 |
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Target: 5'- aUCAGCGCCCCCUGCUCACCACGACGGu -3' miRNA: 3'- -AGUCGCGGGGGACGAGUGGUGCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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