Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21971 | 5' | -57.4 | NC_004914.1 | + | 8808 | 0.66 | 0.57062 |
Target: 5'- gGGCCUGCUggUCCGC-GUGGUgagAAGgGCg -3' miRNA: 3'- aUUGGACGA--GGGUGuCGCCG---UUCgCG- -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 47 | 0.66 | 0.559843 |
Target: 5'- -cGCCUGCauaUCUCAgAGCacGGCAAaauuuGCGCg -3' miRNA: 3'- auUGGACG---AGGGUgUCG--CCGUU-----CGCG- -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 56092 | 0.67 | 0.496626 |
Target: 5'- cAGCCagcGCUCCCGaaaccuuaaAGCGGCA-GCGa -3' miRNA: 3'- aUUGGa--CGAGGGUg--------UCGCCGUuCGCg -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 39100 | 0.69 | 0.399339 |
Target: 5'- cAGCCaGCuUCCCugcCAGCGuuGCGAGUGCu -3' miRNA: 3'- aUUGGaCG-AGGGu--GUCGC--CGUUCGCG- -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 12832 | 0.69 | 0.399339 |
Target: 5'- aAACCUGCcCCgGCGucGUGGCAAcCGCa -3' miRNA: 3'- aUUGGACGaGGgUGU--CGCCGUUcGCG- -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 7019 | 0.69 | 0.390293 |
Target: 5'- -uGCCUGUUCUCcCGGUgaGGUGAGCGUg -3' miRNA: 3'- auUGGACGAGGGuGUCG--CCGUUCGCG- -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 11591 | 0.71 | 0.30672 |
Target: 5'- aGGCCUGUcauauucUCUgGCGGCGGCAGGUa- -3' miRNA: 3'- aUUGGACG-------AGGgUGUCGCCGUUCGcg -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 16846 | 0.73 | 0.232667 |
Target: 5'- uUGGCgCUGgUCUgGguGCGGCAGGUGCa -3' miRNA: 3'- -AUUG-GACgAGGgUguCGCCGUUCGCG- -5' |
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21971 | 5' | -57.4 | NC_004914.1 | + | 26460 | 1.1 | 0.00044 |
Target: 5'- aUAACCUGCUCCCACAGCGGCAAGCGCg -3' miRNA: 3'- -AUUGGACGAGGGUGUCGCCGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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