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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21972 | 5' | -58.8 | NC_004914.1 | + | 8655 | 0.66 | 0.556928 |
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Target: 5'- cUCuCCUGCgccaGCCugGCCUuuUCAGCAu -3' miRNA: 3'- cAGuGGGCGg---CGGugUGGA--AGUCGUc -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 9675 | 0.67 | 0.454469 |
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Target: 5'- gGUCGCCCccaccggGCCuGCCGCACCUguuucuccuuUCGGUc- -3' miRNA: 3'- -CAGUGGG-------CGG-CGGUGUGGA----------AGUCGuc -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 21145 | 0.68 | 0.426937 |
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Target: 5'- cUUGCCCGUgGCaaCGCGCCgaCGGCAGa -3' miRNA: 3'- cAGUGGGCGgCG--GUGUGGaaGUCGUC- -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 21083 | 0.68 | 0.399524 |
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Target: 5'- ---uUCCGuuGCCACAUCUUCAgagaacGCAGg -3' miRNA: 3'- caguGGGCggCGGUGUGGAAGU------CGUC- -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 20557 | 0.69 | 0.364773 |
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Target: 5'- cGUCACCCucuGuuGCCAUAuCCgcaUCAGCAu -3' miRNA: 3'- -CAGUGGG---CggCGGUGU-GGa--AGUCGUc -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 19720 | 0.7 | 0.340115 |
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Target: 5'- aUCACCCaCCuuucuaGCCACACCgcCAGCAa -3' miRNA: 3'- cAGUGGGcGG------CGGUGUGGaaGUCGUc -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 61839 | 0.7 | 0.332169 |
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Target: 5'- uUCACCCGggcCCG-CGCACUUUCAGUAa -3' miRNA: 3'- cAGUGGGC---GGCgGUGUGGAAGUCGUc -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 249 | 0.72 | 0.229299 |
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Target: 5'- aUCACCa--C-CCACGCCUUCAGCAGu -3' miRNA: 3'- cAGUGGgcgGcGGUGUGGAAGUCGUC- -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 35936 | 0.74 | 0.186301 |
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Target: 5'- -aCACCaaagCGCCGCCAUACCgguauugCAGCGGc -3' miRNA: 3'- caGUGG----GCGGCGGUGUGGaa-----GUCGUC- -5' |
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| 21972 | 5' | -58.8 | NC_004914.1 | + | 26677 | 1.07 | 0.000633 |
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Target: 5'- gGUCACCCGCCGCCACACCUUCAGCAGu -3' miRNA: 3'- -CAGUGGGCGGCGGUGUGGAAGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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