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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21973 | 3' | -46.1 | NC_004914.1 | + | 36664 | 0.66 | 0.995592 |
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Target: 5'- uGGAAAGGagaaCCCGG-UGGCGUgauUGCGg -3' miRNA: 3'- -UCUUUUCg---GGGUCaAUUGUAau-GCGC- -5' |
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| 21973 | 3' | -46.1 | NC_004914.1 | + | 48489 | 0.66 | 0.995592 |
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Target: 5'- ----cGGCCUCAGUUAGCcagugaGCGCa -3' miRNA: 3'- ucuuuUCGGGGUCAAUUGuaa---UGCGc -5' |
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| 21973 | 3' | -46.1 | NC_004914.1 | + | 28630 | 0.68 | 0.988673 |
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Target: 5'- uGGAuuGAGCCUCAGaUUAAUGUUAaGCGu -3' miRNA: 3'- -UCUu-UUCGGGGUC-AAUUGUAAUgCGC- -5' |
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| 21973 | 3' | -46.1 | NC_004914.1 | + | 15530 | 0.7 | 0.965395 |
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Target: 5'- uGGAAAGGCCCCgAGUcaacuUAACGUUAaccCGa -3' miRNA: 3'- -UCUUUUCGGGG-UCA-----AUUGUAAUgc-GC- -5' |
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| 21973 | 3' | -46.1 | NC_004914.1 | + | 5666 | 0.73 | 0.883919 |
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Target: 5'- ---uGAGcCCCCAG-UAACAUgGCGCGg -3' miRNA: 3'- ucuuUUC-GGGGUCaAUUGUAaUGCGC- -5' |
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| 21973 | 3' | -46.1 | NC_004914.1 | + | 21504 | 0.76 | 0.736952 |
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Target: 5'- uGAAGGGCUaCCGGcuccUGACAUUGCGCGu -3' miRNA: 3'- uCUUUUCGG-GGUCa---AUUGUAAUGCGC- -5' |
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| 21973 | 3' | -46.1 | NC_004914.1 | + | 28757 | 1.1 | 0.008396 |
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Target: 5'- gAGAAAAGCCCCAGUUAACAUUACGCGu -3' miRNA: 3'- -UCUUUUCGGGGUCAAUUGUAAUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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