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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21985 | 3' | -52.8 | NC_004914.1 | + | 23862 | 0.67 | 0.789183 |
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Target: 5'- uGGCGGagaaaCAGCCGaAUAAAUuCGUGGCGGc -3' miRNA: 3'- -UUGCC-----GUCGGC-UAUUUG-GUAUCGCCu -5' |
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| 21985 | 3' | -52.8 | NC_004914.1 | + | 2109 | 0.67 | 0.758323 |
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Target: 5'- aGGCGGUAGCUGuggcAUCcgGGCGGGu -3' miRNA: 3'- -UUGCCGUCGGCuauuUGGuaUCGCCU- -5' |
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| 21985 | 3' | -52.8 | NC_004914.1 | + | 27552 | 0.69 | 0.648528 |
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Target: 5'- aAAUGGCAgcguauuuGCUGAaAAAgCAUGGCGGAu -3' miRNA: 3'- -UUGCCGU--------CGGCUaUUUgGUAUCGCCU- -5' |
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| 21985 | 3' | -52.8 | NC_004914.1 | + | 24998 | 0.71 | 0.569981 |
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Target: 5'- gGACGGCGGuUCGGggauGACCAacGCGGAa -3' miRNA: 3'- -UUGCCGUC-GGCUau--UUGGUauCGCCU- -5' |
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| 21985 | 3' | -52.8 | NC_004914.1 | + | 47797 | 0.73 | 0.432682 |
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Target: 5'- uGCGGCGGCUGAaaaaugGAACCGgagAGCaGGAg -3' miRNA: 3'- uUGCCGUCGGCUa-----UUUGGUa--UCG-CCU- -5' |
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| 21985 | 3' | -52.8 | NC_004914.1 | + | 25756 | 0.76 | 0.293417 |
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Target: 5'- aGACGGCGG-UGAUGAACCugaaGGCGGAg -3' miRNA: 3'- -UUGCCGUCgGCUAUUUGGua--UCGCCU- -5' |
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| 21985 | 3' | -52.8 | NC_004914.1 | + | 58785 | 1.07 | 0.002289 |
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Target: 5'- gAACGGCAGCCGAUAAACCAUAGCGGAg -3' miRNA: 3'- -UUGCCGUCGGCUAUUUGGUAUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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