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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21997 | 5' | -49.4 | NC_004914.1 | + | 41109 | 0.66 | 0.974008 |
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Target: 5'- cGUAGuCGAGAGUcGA-CGUuguuuaaagaGCCUGCCa -3' miRNA: 3'- -CAUCuGCUUUCA-CUaGCG----------UGGAUGGg -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 59055 | 0.66 | 0.970966 |
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Target: 5'- -cAGACGAAuuaaugcaGGUGAUCGaaaagguGCCUGCg- -3' miRNA: 3'- caUCUGCUU--------UCACUAGCg------UGGAUGgg -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 24110 | 0.66 | 0.970966 |
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Target: 5'- -aGGGCGAgcGUuAUCGUgaugACCUugCCa -3' miRNA: 3'- caUCUGCUuuCAcUAGCG----UGGAugGG- -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 23670 | 0.66 | 0.970966 |
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Target: 5'- -cGGACGAuAGaaUGcgUGCACCcUGCCg -3' miRNA: 3'- caUCUGCUuUC--ACuaGCGUGG-AUGGg -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 38097 | 0.67 | 0.951886 |
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Target: 5'- --cGACGAAAGUaugCGUaccGCCUGCUCu -3' miRNA: 3'- cauCUGCUUUCAcuaGCG---UGGAUGGG- -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 5548 | 0.68 | 0.937114 |
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Target: 5'- ---cACGAAAGaGGUCGCugCUGCaaCCa -3' miRNA: 3'- caucUGCUUUCaCUAGCGugGAUG--GG- -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 53122 | 0.68 | 0.937114 |
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Target: 5'- -cAGACGAAGaUGGUcaaaaCGCGCCUgauagacaucaaGCCCu -3' miRNA: 3'- caUCUGCUUUcACUA-----GCGUGGA------------UGGG- -5' |
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| 21997 | 5' | -49.4 | NC_004914.1 | + | 37631 | 1.14 | 0.002319 |
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Target: 5'- aGUAGACGAAAGUGAUCGCACCUACCCg -3' miRNA: 3'- -CAUCUGCUUUCACUAGCGUGGAUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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