Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2200 | 5' | -63.1 | NC_001402.1 | + | 1081 | 0.67 | 0.010873 |
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Target: 5'- -aGGUGGUGuCGGAGAAGCuCGCcaCCu -3' miRNA: 3'- aaCCGCCGCcGCCUCUUCG-GCGa-GGu -5' |
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| 2200 | 5' | -63.1 | NC_001402.1 | + | 1202 | 0.67 | 0.009392 |
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Target: 5'- -cGGCGGggcCGGCGGgaGGAGGCgGCggccCCGa -3' miRNA: 3'- aaCCGCC---GCCGCC--UCUUCGgCGa---GGU- -5' |
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| 2200 | 5' | -63.1 | NC_001402.1 | + | 940 | 0.7 | 0.004511 |
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Target: 5'- cUGGuCGGCGGUGGAagggaagcagGAGGCgGCggCCAg -3' miRNA: 3'- aACC-GCCGCCGCCU----------CUUCGgCGa-GGU- -5' |
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| 2200 | 5' | -63.1 | NC_001402.1 | + | 1148 | 0.72 | 0.003488 |
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Target: 5'- -aGGCGGCGGCGGGGcc-CCcCUCCc -3' miRNA: 3'- aaCCGCCGCCGCCUCuucGGcGAGGu -5' |
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| 2200 | 5' | -63.1 | NC_001402.1 | + | 787 | 0.72 | 0.003011 |
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Target: 5'- cUGGCGGCGcagcaGCGGAGcagcGAGCUGCagccUCCAg -3' miRNA: 3'- aACCGCCGC-----CGCCUC----UUCGGCG----AGGU- -5' |
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| 2200 | 5' | -63.1 | NC_001402.1 | + | 1075 | 1.05 | 0.000002 |
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Target: 5'- cUUGGCGGCGGCGGAGAAGCCGCUCCAc -3' miRNA: 3'- -AACCGCCGCCGCCUCUUCGGCGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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