miRNA: 3'- -GGUGUCCGcAGuGUUGUGACgAGAga -5'
miRNA: 3'- gGuGUCCGCAGUGUUGugACGagaga -5'
miRNA: 3'- gGUGUCC-GCAGuGuuGUGACGaGAGA- -5'
miRNA: 3'- -GGUGUCCGCAGUGUUGUGACGAGAGA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22002 | 5' | -53.1 | NC_004914.1 | + | 30712 | 0.66 | 0.830317 |
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Target: 5'- cCCGCAGGCuUUuCAGCAUUGgUCUg- -3' miRNA: 3'- -GGUGUCCGcAGuGUUGUGACgAGAga -5' |
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| 22002 | 5' | -53.1 | NC_004914.1 | + | 19331 | 0.68 | 0.751776 |
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Target: 5'- gC-CGGGUGUCGCAACugUGCa---- -3' miRNA: 3'- gGuGUCCGCAGUGUUGugACGagaga -5' |
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| 22002 | 5' | -53.1 | NC_004914.1 | + | 58231 | 0.71 | 0.554924 |
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Target: 5'- gCGCAGGaCGUC-CuuCACUGC-CUCUg -3' miRNA: 3'- gGUGUCC-GCAGuGuuGUGACGaGAGA- -5' |
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| 22002 | 5' | -53.1 | NC_004914.1 | + | 55773 | 1.09 | 0.001824 |
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Target: 5'- gCCACAGGCGUCACAACACUGCUCUCUu -3' miRNA: 3'- -GGUGUCCGCAGUGUUGUGACGAGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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