Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22006 | 3' | -56.8 | NC_004915.1 | + | 630 | 0.66 | 0.164582 |
Target: 5'- aCCCCg--CCUgguCUUUCCAGGUCuaGAGg -3' miRNA: 3'- -GGGGucaGGG---GAAAGGUCUAGggCUC- -5' |
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22006 | 3' | -56.8 | NC_004915.1 | + | 4640 | 0.66 | 0.180527 |
Target: 5'- gCCCCGGcUCCCagcaagUCgGGA-CCCGAu -3' miRNA: 3'- -GGGGUC-AGGGgaa---AGgUCUaGGGCUc -5' |
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22006 | 3' | -56.8 | NC_004915.1 | + | 4250 | 0.67 | 0.140807 |
Target: 5'- gUCCCGGcCCCCgucuucagUUUCGGAgcCCCGAu -3' miRNA: 3'- -GGGGUCaGGGGa-------AAGGUCUa-GGGCUc -5' |
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22006 | 3' | -56.8 | NC_004915.1 | + | 972 | 0.98 | 0.000414 |
Target: 5'- gCCCCAGUCCCCUUcUCAGAUCCCGAGu -3' miRNA: 3'- -GGGGUCAGGGGAAaGGUCUAGGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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