Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22022 | 5' | -58.2 | NC_004927.1 | + | 30694 | 0.67 | 0.576906 |
Target: 5'- gAGCcGGGaGUccgacuCGGAACCGaGAGGGAACg -3' miRNA: 3'- -UUGuCCC-CG------GCCUUGGC-CUCUCUUGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 75842 | 0.67 | 0.535142 |
Target: 5'- gGGCAGGGGgCGGGG--GGAGAGAcgGCc -3' miRNA: 3'- -UUGUCCCCgGCCUUggCCUCUCU--UGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 325 | 0.67 | 0.535142 |
Target: 5'- gGGCAGGGGgCGGGG--GGAGAGAcgGCc -3' miRNA: 3'- -UUGUCCCCgGCCUUggCCUCUCU--UGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 465 | 0.68 | 0.514653 |
Target: 5'- --gGGGGGCCGGAGagaCGGAc-GGACg -3' miRNA: 3'- uugUCCCCGGCCUUg--GCCUcuCUUGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 20325 | 0.69 | 0.445771 |
Target: 5'- cAGCGGcGGCCcc-ACCGGAGGGAGCg -3' miRNA: 3'- -UUGUCcCCGGccuUGGCCUCUCUUGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 75803 | 0.72 | 0.310108 |
Target: 5'- gGGCAGGGGCCGaccGGCCcGAGAGAcggGCa -3' miRNA: 3'- -UUGUCCCCGGCc--UUGGcCUCUCU---UGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 286 | 0.72 | 0.310108 |
Target: 5'- gGGCAGGGGCCGaccGGCCcGAGAGAcggGCa -3' miRNA: 3'- -UUGUCCCCGGCc--UUGGcCUCUCU---UGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 435 | 0.75 | 0.187746 |
Target: 5'- aGACGGGGaGagacggaCGGAACCGGAGAGAcggACg -3' miRNA: 3'- -UUGUCCC-Cg------GCCUUGGCCUCUCU---UGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 75650 | 0.78 | 0.116117 |
Target: 5'- gGACAGGGGaCgGGGGCCGGuuAGAACa -3' miRNA: 3'- -UUGUCCCC-GgCCUUGGCCucUCUUGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 133 | 0.78 | 0.116117 |
Target: 5'- gGACAGGGGaCgGGGGCCGGuuAGAACa -3' miRNA: 3'- -UUGUCCCC-GgCCUUGGCCucUCUUGa -5' |
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22022 | 5' | -58.2 | NC_004927.1 | + | 904 | 1.05 | 0.001267 |
Target: 5'- uAACAGGGGCCGGAACCGGAGAGAACUa -3' miRNA: 3'- -UUGUCCCCGGCCUUGGCCUCUCUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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