Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22024 | 3' | -54 | NC_004927.1 | + | 47542 | 0.66 | 0.866023 |
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Target: 5'- gGCUGacgacCUGUUCCGaCGAGUCgCUGAGa -3' miRNA: 3'- -CGACc----GGCGAGGUaGCUCAGaGAUUC- -5' |
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| 22024 | 3' | -54 | NC_004927.1 | + | 14165 | 0.67 | 0.813294 |
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Target: 5'- gGCUGuuccGCCGCacUCCAcgcaguuUCGAGUCUCc--- -3' miRNA: 3'- -CGAC----CGGCG--AGGU-------AGCUCAGAGauuc -5' |
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| 22024 | 3' | -54 | NC_004927.1 | + | 71771 | 0.67 | 0.775832 |
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Target: 5'- --aGGCCGguaucaCUCCAUCGAGUCg----- -3' miRNA: 3'- cgaCCGGC------GAGGUAGCUCAGagauuc -5' |
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| 22024 | 3' | -54 | NC_004927.1 | + | 8067 | 0.69 | 0.692794 |
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Target: 5'- aGUUGGaaCGCUCauuUUGAGUCUUUAAGa -3' miRNA: 3'- -CGACCg-GCGAGgu-AGCUCAGAGAUUC- -5' |
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| 22024 | 3' | -54 | NC_004927.1 | + | 65175 | 0.7 | 0.660408 |
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Target: 5'- --cGGUCGCgUCgGUCGuGUCUCUGAc -3' miRNA: 3'- cgaCCGGCG-AGgUAGCuCAGAGAUUc -5' |
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| 22024 | 3' | -54 | NC_004927.1 | + | 3572 | 1.12 | 0.001309 |
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Target: 5'- aGCUGGCCGCUCCAUCGAGUCUCUAAGa -3' miRNA: 3'- -CGACCGGCGAGGUAGCUCAGAGAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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