miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22025 5' -53.8 NC_004927.1 + 2286 0.69 0.66606
Target:  5'- -cGUUAGCUUCGcaCGGCUCGAugaugugaaccUCGCg -3'
miRNA:   3'- auCAGUCGGAGUcaGCCGAGUU-----------AGCG- -5'
22025 5' -53.8 NC_004927.1 + 4031 0.69 0.655102
Target:  5'- cGGUCGGCuCUC--UCGGCUCGuUCGa -3'
miRNA:   3'- aUCAGUCG-GAGucAGCCGAGUuAGCg -5'
22025 5' -53.8 NC_004927.1 + 4690 1.11 0.001318
Target:  5'- gUAGUCAGCCUCAGUCGGCUCAAUCGCa -3'
miRNA:   3'- -AUCAGUCGGAGUCAGCCGAGUUAGCG- -5'
22025 5' -53.8 NC_004927.1 + 4889 0.66 0.829525
Target:  5'- gGGUUcGUCUCGGUCGucguaGCUCGAUgCGUc -3'
miRNA:   3'- aUCAGuCGGAGUCAGC-----CGAGUUA-GCG- -5'
22025 5' -53.8 NC_004927.1 + 23749 0.66 0.847124
Target:  5'- --cUCGGCCUCGGUC-GCUUcuUCGa -3'
miRNA:   3'- aucAGUCGGAGUCAGcCGAGuuAGCg -5'
22025 5' -53.8 NC_004927.1 + 38243 0.66 0.838433
Target:  5'- -cGUCGGCgUCA--CGGUguUCGGUCGCc -3'
miRNA:   3'- auCAGUCGgAGUcaGCCG--AGUUAGCG- -5'
22025 5' -53.8 NC_004927.1 + 58475 0.67 0.781088
Target:  5'- uUGGUCGGCCaCGGUaacaguaUGGCagAGUCGUg -3'
miRNA:   3'- -AUCAGUCGGaGUCA-------GCCGagUUAGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.