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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22025 | 5' | -53.8 | NC_004927.1 | + | 2286 | 0.69 | 0.66606 |
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Target: 5'- -cGUUAGCUUCGcaCGGCUCGAugaugugaaccUCGCg -3' miRNA: 3'- auCAGUCGGAGUcaGCCGAGUU-----------AGCG- -5' |
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| 22025 | 5' | -53.8 | NC_004927.1 | + | 4031 | 0.69 | 0.655102 |
|
Target: 5'- cGGUCGGCuCUC--UCGGCUCGuUCGa -3' miRNA: 3'- aUCAGUCG-GAGucAGCCGAGUuAGCg -5' |
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| 22025 | 5' | -53.8 | NC_004927.1 | + | 4690 | 1.11 | 0.001318 |
|
Target: 5'- gUAGUCAGCCUCAGUCGGCUCAAUCGCa -3' miRNA: 3'- -AUCAGUCGGAGUCAGCCGAGUUAGCG- -5' |
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| 22025 | 5' | -53.8 | NC_004927.1 | + | 4889 | 0.66 | 0.829525 |
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Target: 5'- gGGUUcGUCUCGGUCGucguaGCUCGAUgCGUc -3' miRNA: 3'- aUCAGuCGGAGUCAGC-----CGAGUUA-GCG- -5' |
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| 22025 | 5' | -53.8 | NC_004927.1 | + | 23749 | 0.66 | 0.847124 |
|
Target: 5'- --cUCGGCCUCGGUC-GCUUcuUCGa -3' miRNA: 3'- aucAGUCGGAGUCAGcCGAGuuAGCg -5' |
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| 22025 | 5' | -53.8 | NC_004927.1 | + | 38243 | 0.66 | 0.838433 |
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Target: 5'- -cGUCGGCgUCA--CGGUguUCGGUCGCc -3' miRNA: 3'- auCAGUCGgAGUcaGCCG--AGUUAGCG- -5' |
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| 22025 | 5' | -53.8 | NC_004927.1 | + | 58475 | 0.67 | 0.781088 |
|
Target: 5'- uUGGUCGGCCaCGGUaacaguaUGGCagAGUCGUg -3' miRNA: 3'- -AUCAGUCGGaGUCA-------GCCGagUUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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