Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22027 | 3' | -54.4 | NC_004927.1 | + | 7062 | 1.07 | 0.002317 |
Target: 5'- gGACUUGAGCCACACCGAGAGACAGCUu -3' miRNA: 3'- -CUGAACUCGGUGUGGCUCUCUGUCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 57265 | 0.74 | 0.395535 |
Target: 5'- cGACaaaGAGCUACgACgCGAGGGACAGCa -3' miRNA: 3'- -CUGaa-CUCGGUG-UG-GCUCUCUGUCGg -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 6388 | 0.71 | 0.522547 |
Target: 5'- -cUUUGAGCCGC-CaCGAGAGGCGuGCUu -3' miRNA: 3'- cuGAACUCGGUGuG-GCUCUCUGU-CGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 75803 | 0.7 | 0.59717 |
Target: 5'- gGGCagGGGCCGaccggcCCGAGAGACGGgCa -3' miRNA: 3'- -CUGaaCUCGGUgu----GGCUCUCUGUCgG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 286 | 0.7 | 0.59717 |
Target: 5'- gGGCagGGGCCGaccggcCCGAGAGACGGgCa -3' miRNA: 3'- -CUGaaCUCGGUgu----GGCUCUCUGUCgG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 75403 | 0.68 | 0.693601 |
Target: 5'- gGGCUUGAGUgccugaaCGCAUCGAGGGugGuGUCc -3' miRNA: 3'- -CUGAACUCG-------GUGUGGCUCUCugU-CGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 8827 | 0.68 | 0.705348 |
Target: 5'- cGAUaa-AGCCACGCCacGAGAGACugaacuuGCCg -3' miRNA: 3'- -CUGaacUCGGUGUGG--CUCUCUGu------CGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 30698 | 0.67 | 0.778497 |
Target: 5'- -uCUUGAGCCGggaguccgacucggaACCGAGAGggaacGCGGUCg -3' miRNA: 3'- cuGAACUCGGUg--------------UGGCUCUC-----UGUCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 26296 | 0.67 | 0.796969 |
Target: 5'- ----aGAGCCACGucagCGGGAGACuGUCa -3' miRNA: 3'- cugaaCUCGGUGUg---GCUCUCUGuCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 75879 | 0.66 | 0.806453 |
Target: 5'- -----cGGCC-CAuuagaaucCCGAGAGACGGCCc -3' miRNA: 3'- cugaacUCGGuGU--------GGCUCUCUGUCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 69254 | 0.66 | 0.806453 |
Target: 5'- cGCgaGAaCCGCGCCGAGgauGGugAGCUg -3' miRNA: 3'- cUGaaCUcGGUGUGGCUC---UCugUCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 362 | 0.66 | 0.806453 |
Target: 5'- -----cGGCC-CAuuagaaucCCGAGAGACGGCCc -3' miRNA: 3'- cugaacUCGGuGU--------GGCUCUCUGUCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 38535 | 0.66 | 0.833793 |
Target: 5'- aACUUGGuaaucgaCGCAUCGAGGGuuuCGGCCa -3' miRNA: 3'- cUGAACUcg-----GUGUGGCUCUCu--GUCGG- -5' |
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22027 | 3' | -54.4 | NC_004927.1 | + | 46978 | 0.66 | 0.842504 |
Target: 5'- uGACUacgagGAGCCguACACgugCGAGAGcuguCGGCCc -3' miRNA: 3'- -CUGAa----CUCGG--UGUG---GCUCUCu---GUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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