Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22029 | 5' | -55.1 | NC_004927.1 | + | 449 | 0.66 | 0.844804 |
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Target: 5'- --gGACGGACGGGGa-GAgaCGGGGa -3' miRNA: 3'- aagCUGCCUGCCCUacCUaaGCUCCc -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 57098 | 0.67 | 0.761851 |
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Target: 5'- uUUCGccucaGGGCGGGAUGGAgugcuacgUGaAGGGu -3' miRNA: 3'- -AAGCug---CCUGCCCUACCUaa------GC-UCCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 248 | 0.68 | 0.741783 |
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Target: 5'- --aGACGGACGGGcaGGGggCaGGGGa -3' miRNA: 3'- aagCUGCCUGCCCuaCCUaaGcUCCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 75765 | 0.68 | 0.741783 |
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Target: 5'- --aGACGGACGGGcaGGGggCaGGGGa -3' miRNA: 3'- aagCUGCCUGCCCuaCCUaaGcUCCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 42319 | 0.68 | 0.731588 |
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Target: 5'- gUUGGCGGcgACGGGGUaGGcaUCGAGGc -3' miRNA: 3'- aAGCUGCC--UGCCCUA-CCuaAGCUCCc -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 53503 | 0.7 | 0.594215 |
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Target: 5'- --aGACGaAgGGGAUGGAUUCGAcccucguggcGGGg -3' miRNA: 3'- aagCUGCcUgCCCUACCUAAGCU----------CCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 335 | 0.7 | 0.583611 |
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Target: 5'- -cCGuAUGGACGGGcaGGGggCGGGGGg -3' miRNA: 3'- aaGC-UGCCUGCCCuaCCUaaGCUCCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 58772 | 0.7 | 0.583611 |
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Target: 5'- -cCGugGGGCGGuGUGuccucggauagaGAUUCGGGGGu -3' miRNA: 3'- aaGCugCCUGCCcUAC------------CUAAGCUCCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 75852 | 0.7 | 0.583611 |
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Target: 5'- -cCGuAUGGACGGGcaGGGggCGGGGGg -3' miRNA: 3'- aaGC-UGCCUGCCCuaCCUaaGCUCCC- -5' |
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| 22029 | 5' | -55.1 | NC_004927.1 | + | 9062 | 1.07 | 0.00242 |
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Target: 5'- cUUCGACGGACGGGAUGGAUUCGAGGGc -3' miRNA: 3'- -AAGCUGCCUGCCCUACCUAAGCUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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