miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22030 3' -52.5 NC_004927.1 + 10552 1.12 0.002058
Target:  5'- gGGGAACGGAAACAGGUCGUCAGCGAGg -3'
miRNA:   3'- -CCCUUGCCUUUGUCCAGCAGUCGCUC- -5'
22030 3' -52.5 NC_004927.1 + 23119 0.74 0.517973
Target:  5'- aGGGAGCGGGuuCuGGUUGuUCAGCGc- -3'
miRNA:   3'- -CCCUUGCCUuuGuCCAGC-AGUCGCuc -5'
22030 3' -52.5 NC_004927.1 + 31100 0.72 0.668078
Target:  5'- gGGGAACGGAugUucGUCGUCGGgGuGg -3'
miRNA:   3'- -CCCUUGCCUuuGucCAGCAGUCgCuC- -5'
22030 3' -52.5 NC_004927.1 + 57085 0.68 0.852593
Target:  5'- cGGGAUGGAgugcuacgugaaggGugGGGaCGUUGGCGAGc -3'
miRNA:   3'- cCCUUGCCU--------------UugUCCaGCAGUCGCUC- -5'
22030 3' -52.5 NC_004927.1 + 73055 0.66 0.91333
Target:  5'- uGGAGuuCGGAGACGcuGGUauugaGcCAGCGAGg -3'
miRNA:   3'- cCCUU--GCCUUUGU--CCAg----CaGUCGCUC- -5'
22030 3' -52.5 NC_004927.1 + 30611 0.66 0.919448
Target:  5'- --cGACGGAGAC-GGUCugcuUUAGCGAGa -3'
miRNA:   3'- cccUUGCCUUUGuCCAGc---AGUCGCUC- -5'
22030 3' -52.5 NC_004927.1 + 23675 0.66 0.930881
Target:  5'- aGGGAugGGucacGAACAGGcCGUC-GCc-- -3'
miRNA:   3'- -CCCUugCC----UUUGUCCaGCAGuCGcuc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.