Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22030 | 5' | -56 | NC_004927.1 | + | 10516 | 1.05 | 0.002057 |
Target: 5'- gUACUCCCGAAGCCCGUCUCUGUUCCUc -3' miRNA: 3'- -AUGAGGGCUUCGGGCAGAGACAAGGA- -5' |
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22030 | 5' | -56 | NC_004927.1 | + | 25381 | 0.7 | 0.478576 |
Target: 5'- gUACUCCCGAgucucauucuGGUUCGUCgggUGUUCCUu -3' miRNA: 3'- -AUGAGGGCU----------UCGGGCAGag-ACAAGGA- -5' |
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22030 | 5' | -56 | NC_004927.1 | + | 12197 | 0.68 | 0.614785 |
Target: 5'- aUGCUCCgGggGUUCGUCUCgaaucgCCUc -3' miRNA: 3'- -AUGAGGgCuuCGGGCAGAGacaa--GGA- -5' |
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22030 | 5' | -56 | NC_004927.1 | + | 6572 | 0.66 | 0.762866 |
Target: 5'- -cCUCUCGGAGUUCGUCgaugcGUUCCUc -3' miRNA: 3'- auGAGGGCUUCGGGCAGaga--CAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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