miRNA: 3'- -CGAACaa-GGgUg---CCG-AGGU-CUACUUG- -5'
miRNA: 3'- -CGaacaAGGgUGCCGAGGUCua-CUUG- -5'
miRNA: 3'- cgaACaAGGGuGCCGaGGUCUACUUG- -5'
miRNA: 3'- cgaaCAAGGGu----GCCG-AGgUCUACUUG- -5'
miRNA: 3'- -CGAACAAGGGUGCCGAGGUCUACUUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22031 | 3' | -51.9 | NC_004927.1 | + | 74011 | 0.66 | 0.922183 |
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Target: 5'- gGCUUGacgCCgAaggaGGCgUCCAaGAUGAACa -3' miRNA: 3'- -CGAACaa-GGgUg---CCG-AGGU-CUACUUG- -5' |
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| 22031 | 3' | -51.9 | NC_004927.1 | + | 22820 | 0.66 | 0.909653 |
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Target: 5'- gGCg---UCCgGCGGCUUCAGcucGAACg -3' miRNA: 3'- -CGaacaAGGgUGCCGAGGUCua-CUUG- -5' |
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| 22031 | 3' | -51.9 | NC_004927.1 | + | 67780 | 0.67 | 0.873556 |
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Target: 5'- --cUGcUCCCuCGGCgUCGGGUGAGCu -3' miRNA: 3'- cgaACaAGGGuGCCGaGGUCUACUUG- -5' |
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| 22031 | 3' | -51.9 | NC_004927.1 | + | 4388 | 0.68 | 0.8401 |
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Target: 5'- ---cGUUCCUuacguCGGCaUCgAGAUGGACg -3' miRNA: 3'- cgaaCAAGGGu----GCCG-AGgUCUACUUG- -5' |
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| 22031 | 3' | -51.9 | NC_004927.1 | + | 11507 | 1.12 | 0.002024 |
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Target: 5'- cGCUUGUUCCCACGGCUCCAGAUGAACc -3' miRNA: 3'- -CGAACAAGGGUGCCGAGGUCUACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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