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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22032 | 3' | -59.5 | NC_004927.1 | + | 59583 | 0.66 | 0.599819 |
|
Target: 5'- gGGGCCGCGAGCGuaaucUUCGgCCUcaaguCGGAa -3' miRNA: 3'- -CCCGGUGCUUGCc----GAGCaGGGu----GCCU- -5' |
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| 22032 | 3' | -59.5 | NC_004927.1 | + | 49184 | 0.66 | 0.641106 |
|
Target: 5'- aGGCCGCGAGCuGUaCG-CCCACuacgaGGAc -3' miRNA: 3'- cCCGGUGCUUGcCGaGCaGGGUG-----CCU- -5' |
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| 22032 | 3' | -59.5 | NC_004927.1 | + | 67090 | 0.67 | 0.528755 |
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Target: 5'- -cGCCGCuGACGaGCuUCGUCaCCACGGc -3' miRNA: 3'- ccCGGUGcUUGC-CG-AGCAG-GGUGCCu -5' |
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| 22032 | 3' | -59.5 | NC_004927.1 | + | 14788 | 0.67 | 0.558915 |
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Target: 5'- aGGCUAUGucCGGCUUGcCgUCGCGGAg -3' miRNA: 3'- cCCGGUGCuuGCCGAGCaG-GGUGCCU- -5' |
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| 22032 | 3' | -59.5 | NC_004927.1 | + | 73635 | 0.68 | 0.479936 |
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Target: 5'- gGGGCCGCGAggucgGCGGUUCaGcCCUGCuGGu -3' miRNA: 3'- -CCCGGUGCU-----UGCCGAG-CaGGGUG-CCu -5' |
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| 22032 | 3' | -59.5 | NC_004927.1 | + | 12080 | 1.1 | 0.000583 |
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Target: 5'- cGGGCCACGAACGGCUCGUCCCACGGAc -3' miRNA: 3'- -CCCGGUGCUUGCCGAGCAGGGUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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