Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22033 | 3' | -52.5 | NC_004927.1 | + | 71770 | 0.67 | 0.848671 |
Target: 5'- -gGCCGguaucacucCAUCGAgUCGGCAGAAcccGGCg -3' miRNA: 3'- ugCGGCa--------GUGGUUgAGCCGUCUU---UUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 68055 | 0.66 | 0.888508 |
Target: 5'- uCGUCGUCGCCGccgacgcagAUUCGGCA---AACg -3' miRNA: 3'- uGCGGCAGUGGU---------UGAGCCGUcuuUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 66172 | 0.67 | 0.873318 |
Target: 5'- -gGCUaUCgACCGACUCGgGCAGGAGGu -3' miRNA: 3'- ugCGGcAG-UGGUUGAGC-CGUCUUUUg -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 56713 | 0.73 | 0.557537 |
Target: 5'- cUGuuGUUAUCaAGCUCGGCGGAGAGg -3' miRNA: 3'- uGCggCAGUGG-UUGAGCCGUCUUUUg -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 33429 | 0.67 | 0.881042 |
Target: 5'- cACGCCGUCguaAUCGAgUCGGaAGuAAACg -3' miRNA: 3'- -UGCGGCAG---UGGUUgAGCCgUCuUUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 30693 | 0.66 | 0.909312 |
Target: 5'- -aGCCGggaguCCGACUCGGaaccgagAGGGAACg -3' miRNA: 3'- ugCGGCagu--GGUUGAGCCg------UCUUUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 26304 | 0.68 | 0.839989 |
Target: 5'- aACGUCGUagaGCCAcgUCaGCGGGAGACu -3' miRNA: 3'- -UGCGGCAg--UGGUugAGcCGUCUUUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 23240 | 0.77 | 0.352316 |
Target: 5'- uCGCCGUCuCC-GCUCGGUcggGGAGAGCg -3' miRNA: 3'- uGCGGCAGuGGuUGAGCCG---UCUUUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 23184 | 0.69 | 0.753159 |
Target: 5'- aGCGCC---ACCGAC-CGGCAGAAGu- -3' miRNA: 3'- -UGCGGcagUGGUUGaGCCGUCUUUug -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 17944 | 0.67 | 0.848671 |
Target: 5'- aAUGCCGUcCACggaaGAC-CGGCgAGAAGGCu -3' miRNA: 3'- -UGCGGCA-GUGg---UUGaGCCG-UCUUUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 14937 | 0.69 | 0.763452 |
Target: 5'- aACGuauCCGUCACCGuacucgUUCGGCGGGucGCa -3' miRNA: 3'- -UGC---GGCAGUGGUu-----GAGCCGUCUuuUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 12766 | 1.09 | 0.002495 |
Target: 5'- aACGCCGUCACCAACUCGGCAGAAAACu -3' miRNA: 3'- -UGCGGCAGUGGUUGAGCCGUCUUUUG- -5' |
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22033 | 3' | -52.5 | NC_004927.1 | + | 11980 | 0.67 | 0.881042 |
Target: 5'- -gGuuGUCACCGAgguaUgGGUAGGGAACc -3' miRNA: 3'- ugCggCAGUGGUUg---AgCCGUCUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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