miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22033 5' -52.7 NC_004927.1 + 12731 1.1 0.002254
Target:  5'- gAGGUUCCACGCUUCGAGCUGUUCGAGg -3'
miRNA:   3'- -UCCAAGGUGCGAAGCUCGACAAGCUC- -5'
22033 5' -52.7 NC_004927.1 + 29964 0.74 0.499191
Target:  5'- ---aUCUACGC--CGAGCUGUUCGGGg -3'
miRNA:   3'- uccaAGGUGCGaaGCUCGACAAGCUC- -5'
22033 5' -52.7 NC_004927.1 + 62262 0.73 0.541195
Target:  5'- ---cUCUGCGCUUCGAccucGUUGUUCGAGa -3'
miRNA:   3'- uccaAGGUGCGAAGCU----CGACAAGCUC- -5'
22033 5' -52.7 NC_004927.1 + 24237 0.68 0.837502
Target:  5'- aAGGUcacucaucgagcguuUCCACGCaUCGAGg-GUUCGGu -3'
miRNA:   3'- -UCCA---------------AGGUGCGaAGCUCgaCAAGCUc -5'
22033 5' -52.7 NC_004927.1 + 33762 0.66 0.893418
Target:  5'- gAGGcacUCC-CGCUgguucaUCGAGCUGUcaaacucgauauaguUCGAGu -3'
miRNA:   3'- -UCCa--AGGuGCGA------AGCUCGACA---------------AGCUC- -5'
22033 5' -52.7 NC_004927.1 + 42113 0.66 0.911017
Target:  5'- aGGGUUcCCACGagUCGGGCguuucgUCGGGu -3'
miRNA:   3'- -UCCAA-GGUGCgaAGCUCGaca---AGCUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.