Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22034 | 5' | -55.1 | NC_004927.1 | + | 49280 | 0.66 | 0.812688 |
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Target: 5'- --gGGGUCGUGGGuucuucuaAGCGaggaCGCU-CCCg -3' miRNA: 3'- uagCUCAGCACCC--------UUGUg---GCGAaGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 22198 | 0.66 | 0.812688 |
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Target: 5'- -cCGGGUCGUcaacGGGGuCGCCG-UUCUCg -3' miRNA: 3'- uaGCUCAGCA----CCCUuGUGGCgAAGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 14610 | 0.66 | 0.803431 |
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Target: 5'- cUCGuGGUCGUGGGAGCGgucuggaGCgUUCCa -3' miRNA: 3'- uAGC-UCAGCACCCUUGUgg-----CGaAGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 65479 | 0.67 | 0.754778 |
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Target: 5'- --gGAGUgGUGGGAACgACCcCgaCCCg -3' miRNA: 3'- uagCUCAgCACCCUUG-UGGcGaaGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 18501 | 0.67 | 0.754778 |
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Target: 5'- -aCGAGUCG-GGaGGcCACCGCgaccUCCUc -3' miRNA: 3'- uaGCUCAGCaCC-CUuGUGGCGa---AGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 58449 | 0.69 | 0.639108 |
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Target: 5'- --aGAGUCGUGgcGGAACGCUcagGCUcagcucUCCCa -3' miRNA: 3'- uagCUCAGCAC--CCUUGUGG---CGA------AGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 41584 | 0.71 | 0.543182 |
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Target: 5'- cGUCG-GUCGUcGGGGAgagucCACuCGCUUCUCa -3' miRNA: 3'- -UAGCuCAGCA-CCCUU-----GUG-GCGAAGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 39686 | 0.74 | 0.370601 |
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Target: 5'- cAUCGAGUCG-GGGAACGucCCGauaCCCg -3' miRNA: 3'- -UAGCUCAGCaCCCUUGU--GGCgaaGGG- -5' |
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| 22034 | 5' | -55.1 | NC_004927.1 | + | 12846 | 1.11 | 0.001263 |
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Target: 5'- cAUCGAGUCGUGGGAACACCGCUUCCCg -3' miRNA: 3'- -UAGCUCAGCACCCUUGUGGCGAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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