Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22037 | 3' | -53.5 | NC_004927.1 | + | 15098 | 1.11 | 0.001829 |
Target: 5'- uCUCUGCUAUCUCGGUGGACUCGUCGCc -3' miRNA: 3'- -GAGACGAUAGAGCCACCUGAGCAGCG- -5' |
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22037 | 3' | -53.5 | NC_004927.1 | + | 57708 | 0.7 | 0.70584 |
Target: 5'- gCUCUGCUgGUCggUGGUGGAUaCcUCGCu -3' miRNA: 3'- -GAGACGA-UAGa-GCCACCUGaGcAGCG- -5' |
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22037 | 3' | -53.5 | NC_004927.1 | + | 4551 | 0.67 | 0.833709 |
Target: 5'- ---cGCcgGUCgUCGGuUGGugUCGUUGCu -3' miRNA: 3'- gagaCGa-UAG-AGCC-ACCugAGCAGCG- -5' |
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22037 | 3' | -53.5 | NC_004927.1 | + | 9888 | 0.66 | 0.867101 |
Target: 5'- gUC-GCUGUCgcugUCGGUgGGGCUgGUUGUg -3' miRNA: 3'- gAGaCGAUAG----AGCCA-CCUGAgCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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