Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22038 | 3' | -52.5 | NC_004927.1 | + | 27170 | 0.66 | 0.93003 |
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Target: 5'- cGgUGCGAaguaGCCGGAGuGCU-UGCCGUc- -3' miRNA: 3'- -CgAUGCU----UGGCCUC-UGAuGCGGCAuc -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 20324 | 0.68 | 0.845317 |
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Target: 5'- aGCgGCGGccccACCGGAGGgaGCGUCGUc- -3' miRNA: 3'- -CGaUGCU----UGGCCUCUgaUGCGGCAuc -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 20985 | 0.68 | 0.827849 |
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Target: 5'- aGCUACG-GCauaGGuauUUGCGCCGUAGg -3' miRNA: 3'- -CGAUGCuUGg--CCucuGAUGCGGCAUC- -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 51905 | 0.68 | 0.827849 |
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Target: 5'- cCUACGAACCGaaccacGAGcUUugGUCGUGGa -3' miRNA: 3'- cGAUGCUUGGC------CUCuGAugCGGCAUC- -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 57257 | 0.69 | 0.800169 |
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Target: 5'- aGCUACGAcgCGaGGGACagcaAUGCCGUGGu -3' miRNA: 3'- -CGAUGCUugGC-CUCUGa---UGCGGCAUC- -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 46993 | 0.73 | 0.558108 |
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Target: 5'- -gUACGAcCCGGAGACUGacuacgaggaGCCGUAc -3' miRNA: 3'- cgAUGCUuGGCCUCUGAUg---------CGGCAUc -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 75733 | 0.74 | 0.536928 |
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Target: 5'- aGCUACGAACaggcaGGcAGGCaggUACGCgCGUAGg -3' miRNA: 3'- -CGAUGCUUGg----CC-UCUG---AUGCG-GCAUC- -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 216 | 0.74 | 0.536928 |
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Target: 5'- aGCUACGAACaggcaGGcAGGCaggUACGCgCGUAGg -3' miRNA: 3'- -CGAUGCUUGg----CC-UCUG---AUGCG-GCAUC- -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 29094 | 0.74 | 0.516033 |
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Target: 5'- --aGCGGGCCGGuGuCUAUGUCGUAGu -3' miRNA: 3'- cgaUGCUUGGCCuCuGAUGCGGCAUC- -5' |
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| 22038 | 3' | -52.5 | NC_004927.1 | + | 16477 | 1.12 | 0.002099 |
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Target: 5'- aGCUACGAACCGGAGACUACGCCGUAGa -3' miRNA: 3'- -CGAUGCUUGGCCUCUGAUGCGGCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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