Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22041 | 5' | -54.3 | NC_004927.1 | + | 52480 | 0.66 | 0.876846 |
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Target: 5'- aUGGCGGCuaUGAcGCUCUugAcUCcgaGGGc -3' miRNA: 3'- gGCCGCCG--ACUaCGAGAugUuAG---CCC- -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 14238 | 0.67 | 0.809634 |
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Target: 5'- aCCGGgGGUUGuccAUGaacgUCgugucGCAAUCGGGg -3' miRNA: 3'- -GGCCgCCGAC---UACg---AGa----UGUUAGCCC- -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 71733 | 0.69 | 0.731219 |
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Target: 5'- gCGGUGGCUGA-GCgaaucucucaggUCUACAA-CGGa -3' miRNA: 3'- gGCCGCCGACUaCG------------AGAUGUUaGCCc -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 42825 | 0.69 | 0.710464 |
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Target: 5'- -aGGuCGGUgaGGUGUUCUuccCAAUCGGGg -3' miRNA: 3'- ggCC-GCCGa-CUACGAGAu--GUUAGCCC- -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 22816 | 0.69 | 0.710464 |
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Target: 5'- uCCGGCGGCUucaGCUCgaACGAcUCGGu -3' miRNA: 3'- -GGCCGCCGAcuaCGAGa-UGUU-AGCCc -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 72619 | 0.7 | 0.657477 |
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Target: 5'- gUCGGCGGaUUGGUGUUucCUACAuaAUCGGu -3' miRNA: 3'- -GGCCGCC-GACUACGA--GAUGU--UAGCCc -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 67798 | 0.71 | 0.613552 |
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Target: 5'- gCGGCGGCgUGAcgcagacUGCUCccucgGCG-UCGGGu -3' miRNA: 3'- gGCCGCCG-ACU-------ACGAGa----UGUuAGCCC- -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 50325 | 0.72 | 0.529071 |
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Target: 5'- -aGGCGGCgGAgcagguggcagucUGCUCUcgaAAUCGGGg -3' miRNA: 3'- ggCCGCCGaCU-------------ACGAGAug-UUAGCCC- -5' |
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| 22041 | 5' | -54.3 | NC_004927.1 | + | 16941 | 1.13 | 0.001137 |
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Target: 5'- uCCGGCGGCUGAUGCUCUACAAUCGGGg -3' miRNA: 3'- -GGCCGCCGACUACGAGAUGUUAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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