miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22042 3' -60.2 NC_004927.1 + 8389 0.66 0.583923
Target:  5'- aCCGCCGGUUguaggagaacaCCUua-CC-UCCCACCGa -3'
miRNA:   3'- -GGUGGCCGA-----------GGGuacGGuAGGGUGGC- -5'
22042 3' -60.2 NC_004927.1 + 24020 0.66 0.582897
Target:  5'- uUCACCGGCUCUUguaucuaaaccggGUGCUugCCCuCCGu -3'
miRNA:   3'- -GGUGGCCGAGGG-------------UACGGuaGGGuGGC- -5'
22042 3' -60.2 NC_004927.1 + 10882 0.66 0.573674
Target:  5'- gCCGCUGGCUCCacagAUGUaGUCCUGUCGg -3'
miRNA:   3'- -GGUGGCCGAGGg---UACGgUAGGGUGGC- -5'
22042 3' -60.2 NC_004927.1 + 9611 0.67 0.522198
Target:  5'- gUACCGGaCUCCCuUGCCGUggagaggCCCuuCGa -3'
miRNA:   3'- gGUGGCC-GAGGGuACGGUA-------GGGugGC- -5'
22042 3' -60.2 NC_004927.1 + 35189 0.69 0.367275
Target:  5'- gCGUCGGCUCCCGUGaaGUCCUcggcgggGCCGu -3'
miRNA:   3'- gGUGGCCGAGGGUACggUAGGG-------UGGC- -5'
22042 3' -60.2 NC_004927.1 + 18786 1.1 0.000478
Target:  5'- uCCACCGGCUCCCAUGCCAUCCCACCGa -3'
miRNA:   3'- -GGUGGCCGAGGGUACGGUAGGGUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.