Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22042 | 3' | -60.2 | NC_004927.1 | + | 18786 | 1.1 | 0.000478 |
Target: 5'- uCCACCGGCUCCCAUGCCAUCCCACCGa -3' miRNA: 3'- -GGUGGCCGAGGGUACGGUAGGGUGGC- -5' |
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22042 | 3' | -60.2 | NC_004927.1 | + | 35189 | 0.69 | 0.367275 |
Target: 5'- gCGUCGGCUCCCGUGaaGUCCUcggcgggGCCGu -3' miRNA: 3'- gGUGGCCGAGGGUACggUAGGG-------UGGC- -5' |
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22042 | 3' | -60.2 | NC_004927.1 | + | 9611 | 0.67 | 0.522198 |
Target: 5'- gUACCGGaCUCCCuUGCCGUggagaggCCCuuCGa -3' miRNA: 3'- gGUGGCC-GAGGGuACGGUA-------GGGugGC- -5' |
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22042 | 3' | -60.2 | NC_004927.1 | + | 10882 | 0.66 | 0.573674 |
Target: 5'- gCCGCUGGCUCCacagAUGUaGUCCUGUCGg -3' miRNA: 3'- -GGUGGCCGAGGg---UACGgUAGGGUGGC- -5' |
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22042 | 3' | -60.2 | NC_004927.1 | + | 24020 | 0.66 | 0.582897 |
Target: 5'- uUCACCGGCUCUUguaucuaaaccggGUGCUugCCCuCCGu -3' miRNA: 3'- -GGUGGCCGAGGG-------------UACGGuaGGGuGGC- -5' |
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22042 | 3' | -60.2 | NC_004927.1 | + | 8389 | 0.66 | 0.583923 |
Target: 5'- aCCGCCGGUUguaggagaacaCCUua-CC-UCCCACCGa -3' miRNA: 3'- -GGUGGCCGA-----------GGGuacGGuAGGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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