miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22045 5' -60.3 NC_004927.1 + 22282 1.08 0.000596
Target:  5'- uUCGGACGUGCUUCUCCCACCGCCCCAu -3'
miRNA:   3'- -AGCCUGCACGAAGAGGGUGGCGGGGU- -5'
22045 5' -60.3 NC_004927.1 + 28571 0.7 0.337937
Target:  5'- gCGGAUGUgagguucgagccauuGCgcccaaucCUCCCACgGCCCCAu -3'
miRNA:   3'- aGCCUGCA---------------CGaa------GAGGGUGgCGGGGU- -5'
22045 5' -60.3 NC_004927.1 + 6373 0.69 0.350446
Target:  5'- -aGaGGCGUGCUucaUCUCCCACUuCCCUc -3'
miRNA:   3'- agC-CUGCACGA---AGAGGGUGGcGGGGu -5'
22045 5' -60.3 NC_004927.1 + 35563 0.68 0.435816
Target:  5'- aCGG-CGaGCUUCUUCCcguuacgaACCGCUCCGa -3'
miRNA:   3'- aGCCuGCaCGAAGAGGG--------UGGCGGGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.