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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22046 | 3' | -56.5 | NC_004927.1 | + | 22571 | 1.14 | 0.000514 |
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Target: 5'- aGCCGCGUCCACCACCAUGUCCUUGCCa -3' miRNA: 3'- -CGGCGCAGGUGGUGGUACAGGAACGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 15265 | 0.74 | 0.325296 |
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Target: 5'- cCCGagGUCUuCCGCaAUGUCCUUGCCg -3' miRNA: 3'- cGGCg-CAGGuGGUGgUACAGGAACGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 28622 | 0.71 | 0.463534 |
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Target: 5'- uGCCGCGUcaagagccaacucCCACCGCUua-UCCUcgaacUGCCa -3' miRNA: 3'- -CGGCGCA-------------GGUGGUGGuacAGGA-----ACGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 2505 | 0.71 | 0.464499 |
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Target: 5'- cGUCGCuGUugaugggaaCCAUCACgGUGUCaCUUGCCu -3' miRNA: 3'- -CGGCG-CA---------GGUGGUGgUACAG-GAACGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 21096 | 0.68 | 0.628913 |
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Target: 5'- cGCCGC-UCgCGCCGCUGUuGUUgUUGCUa -3' miRNA: 3'- -CGGCGcAG-GUGGUGGUA-CAGgAACGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 40153 | 0.67 | 0.671326 |
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Target: 5'- aGCCGCGUUCgaGCCGCUGUaUCaa-GCCg -3' miRNA: 3'- -CGGCGCAGG--UGGUGGUAcAGgaaCGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 26055 | 0.66 | 0.73371 |
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Target: 5'- uGCCGCGUUC-UgACCGUG-CCggauguggGCCu -3' miRNA: 3'- -CGGCGCAGGuGgUGGUACaGGaa------CGG- -5' |
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| 22046 | 3' | -56.5 | NC_004927.1 | + | 15052 | 0.66 | 0.73371 |
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Target: 5'- cGCCGCGacugCCGggcaacagcauCCACCAacucGUCCUUuCCg -3' miRNA: 3'- -CGGCGCa---GGU-----------GGUGGUa---CAGGAAcGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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