Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22046 | 5' | -56.8 | NC_004927.1 | + | 21331 | 0.66 | 0.705469 |
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Target: 5'- gGAGcGCCGuuGUCGUcGGUGGCGAACCa-- -3' miRNA: 3'- -CUC-CGGC--CAGUA-UCGCUGCUUGGgag -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 20351 | 0.67 | 0.673803 |
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Target: 5'- cGGGCCGGuugguUCAUcgaaggagacAGCGGCGGcCCCa- -3' miRNA: 3'- cUCCGGCC-----AGUA----------UCGCUGCUuGGGag -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 33846 | 0.67 | 0.637523 |
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Target: 5'- cGGGUCGGUCGgcaucccguacucGGCGGCGAggucaacguACaCCUCg -3' miRNA: 3'- cUCCGGCCAGUa------------UCGCUGCU---------UG-GGAG- -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 33165 | 0.67 | 0.631105 |
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Target: 5'- cGGGUuggCGGUCGgccCGGCGuAGCCCUCg -3' miRNA: 3'- cUCCG---GCCAGUaucGCUGC-UUGGGAG- -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 34509 | 0.68 | 0.588415 |
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Target: 5'- cGAGuCCGGUCu--GCGACuguCCCUCa -3' miRNA: 3'- -CUCcGGCCAGuauCGCUGcuuGGGAG- -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 69862 | 0.7 | 0.475187 |
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Target: 5'- --cGCCGGgCGUGGCGACaggguacaGAACCCUg -3' miRNA: 3'- cucCGGCCaGUAUCGCUG--------CUUGGGAg -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 32177 | 0.71 | 0.436687 |
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Target: 5'- cGAGGCCGGUCuugucGGUGucuuCGuACCCUUc -3' miRNA: 3'- -CUCCGGCCAGua---UCGCu---GCuUGGGAG- -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 43187 | 0.72 | 0.357099 |
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Target: 5'- cGGGCCGuGUCGUacAGCGGCauccGAACCCg- -3' miRNA: 3'- cUCCGGC-CAGUA--UCGCUG----CUUGGGag -5' |
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| 22046 | 5' | -56.8 | NC_004927.1 | + | 22536 | 1.08 | 0.001138 |
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Target: 5'- aGAGGCCGGUCAUAGCGACGAACCCUCc -3' miRNA: 3'- -CUCCGGCCAGUAUCGCUGCUUGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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