Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22055 | 3' | -57.2 | NC_004927.1 | + | 32388 | 0.66 | 0.71542 |
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Target: 5'- --aCACGGGgcgGCGCgUCCAucucAAUCGGcUCa -3' miRNA: 3'- ccgGUGCCC---UGCGgAGGU----UUAGCC-AG- -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 28764 | 0.66 | 0.71542 |
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Target: 5'- -uCCACGGGAUGUUUCCGA--UGGg- -3' miRNA: 3'- ccGGUGCCCUGCGGAGGUUuaGCCag -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 5083 | 0.66 | 0.714392 |
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Target: 5'- uGCCGCuGGugGuucgucgucguccCCUCCGucccUCGGUCg -3' miRNA: 3'- cCGGUGcCCugC-------------GGAGGUuu--AGCCAG- -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 63301 | 0.66 | 0.705107 |
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Target: 5'- aGGCCGUGGG-CGaCCUCCGGuaaUGGUg -3' miRNA: 3'- -CCGGUGCCCuGC-GGAGGUUua-GCCAg -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 66808 | 0.66 | 0.694729 |
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Target: 5'- -cCCACGGGugGCCccgcgaCCGAGcCGGg- -3' miRNA: 3'- ccGGUGCCCugCGGa-----GGUUUaGCCag -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 12226 | 0.66 | 0.694729 |
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Target: 5'- cGCCACGGuGCgGCUUCCGGGUUGuUCc -3' miRNA: 3'- cCGGUGCCcUG-CGGAGGUUUAGCcAG- -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 8208 | 0.67 | 0.663298 |
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Target: 5'- aGGCUuCGGGuCGUCg--GGGUCGGUCa -3' miRNA: 3'- -CCGGuGCCCuGCGGaggUUUAGCCAG- -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 59413 | 0.68 | 0.578951 |
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Target: 5'- gGGCgGCGGaGGuCGCaCUCCAAAcUCGG-Ca -3' miRNA: 3'- -CCGgUGCC-CU-GCG-GAGGUUU-AGCCaG- -5' |
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| 22055 | 3' | -57.2 | NC_004927.1 | + | 30723 | 1.11 | 0.00078 |
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Target: 5'- gGGCCACGGGACGCCUCCAAAUCGGUCu -3' miRNA: 3'- -CCGGUGCCCUGCGGAGGUUUAGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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