Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22056 | 5' | -51.4 | NC_004927.1 | + | 45556 | 0.66 | 0.950312 |
Target: 5'- gUGACGGCucccgCGACC---AGCAGGu -3' miRNA: 3'- gACUGCCGcaaa-GCUGGcuuUCGUCU- -5' |
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22056 | 5' | -51.4 | NC_004927.1 | + | 66183 | 0.68 | 0.891995 |
Target: 5'- uCUGuucacuCGGCua-UCGACCGAcucGGGCAGGa -3' miRNA: 3'- -GACu-----GCCGcaaAGCUGGCU---UUCGUCU- -5' |
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22056 | 5' | -51.4 | NC_004927.1 | + | 57554 | 0.72 | 0.694917 |
Target: 5'- uUGACGGCc---CGACCGA--GCAGAa -3' miRNA: 3'- gACUGCCGcaaaGCUGGCUuuCGUCU- -5' |
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22056 | 5' | -51.4 | NC_004927.1 | + | 72127 | 0.72 | 0.705724 |
Target: 5'- -aGACGGaCGUUUCG-CUGuucGAGCAGGa -3' miRNA: 3'- gaCUGCC-GCAAAGCuGGCu--UUCGUCU- -5' |
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22056 | 5' | -51.4 | NC_004927.1 | + | 31225 | 1.1 | 0.003463 |
Target: 5'- gCUGACGGCGUUUCGACCGAAAGCAGAg -3' miRNA: 3'- -GACUGCCGCAAAGCUGGCUUUCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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