Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22061 | 5' | -59.2 | NC_004927.1 | + | 56718 | 0.67 | 0.544706 |
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Target: 5'- -uCGUCCUGUuguuaucaaGCUCGGCGGaGAGGc- -3' miRNA: 3'- ccGUAGGGCA---------UGAGCCGCCgCUCCag -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 2340 | 0.69 | 0.419783 |
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Target: 5'- uGGCGg--UGUAgUCGGUGGCGAGGa- -3' miRNA: 3'- -CCGUaggGCAUgAGCCGCCGCUCCag -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 14930 | 0.71 | 0.352046 |
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Target: 5'- -cCGUCaCCGUACUCGuuCGGCG-GGUCg -3' miRNA: 3'- ccGUAG-GGCAUGAGCc-GCCGCuCCAG- -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 60886 | 0.71 | 0.343359 |
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Target: 5'- gGGCGUCucaacagCCGUGCggugCGuGCGGCGcuugaGGGUCu -3' miRNA: 3'- -CCGUAG-------GGCAUGa---GC-CGCCGC-----UCCAG- -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 35185 | 0.73 | 0.246989 |
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Target: 5'- cGGC-UCCCGUGaagucCUCGGCGGgGccGUCg -3' miRNA: 3'- -CCGuAGGGCAU-----GAGCCGCCgCucCAG- -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 54029 | 0.75 | 0.187553 |
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Target: 5'- uGCggCCCGcuCUCGG-GGCGAGGUCa -3' miRNA: 3'- cCGuaGGGCauGAGCCgCCGCUCCAG- -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 59387 | 0.76 | 0.160735 |
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Target: 5'- cGGCAcaCCCGaGCgUCGGCGGCGGuaauGGUCg -3' miRNA: 3'- -CCGUa-GGGCaUG-AGCCGCCGCU----CCAG- -5' |
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| 22061 | 5' | -59.2 | NC_004927.1 | + | 33837 | 1.12 | 0.000431 |
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Target: 5'- cGGCAUCCCGUACUCGGCGGCGAGGUCa -3' miRNA: 3'- -CCGUAGGGCAUGAGCCGCCGCUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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