Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22062 | 5' | -57.4 | NC_004927.1 | + | 27844 | 0.66 | 0.67319 |
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Target: 5'- aGUUGCUUGcgaGGUugaCGUAGCCGAGuCGGUc -3' miRNA: 3'- gCAGUGGAC---UCG---GUAUCGGCUC-GCCG- -5' |
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| 22062 | 5' | -57.4 | NC_004927.1 | + | 70654 | 0.67 | 0.652038 |
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Target: 5'- aCG-CGCUgGAGCC---GCCGGGUGGUg -3' miRNA: 3'- -GCaGUGGaCUCGGuauCGGCUCGCCG- -5' |
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| 22062 | 5' | -57.4 | NC_004927.1 | + | 42206 | 0.68 | 0.56745 |
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Target: 5'- cCGUCGCUUuGGCgAguaggAGCCGAGCGagaaGCg -3' miRNA: 3'- -GCAGUGGAcUCGgUa----UCGGCUCGC----CG- -5' |
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| 22062 | 5' | -57.4 | NC_004927.1 | + | 46503 | 0.7 | 0.425767 |
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Target: 5'- aGUU-CCUGAGCgacaagcuggaggaCGUAGCC-AGCGGCg -3' miRNA: 3'- gCAGuGGACUCG--------------GUAUCGGcUCGCCG- -5' |
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| 22062 | 5' | -57.4 | NC_004927.1 | + | 21826 | 0.71 | 0.392588 |
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Target: 5'- uCGUCACCgccgGAGUCGccaCCGAGCGaGCc -3' miRNA: 3'- -GCAGUGGa---CUCGGUaucGGCUCGC-CG- -5' |
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| 22062 | 5' | -57.4 | NC_004927.1 | + | 29706 | 0.76 | 0.204471 |
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Target: 5'- gGUCGCCUGAaUCAUGGCgaGAGgGGCg -3' miRNA: 3'- gCAGUGGACUcGGUAUCGg-CUCgCCG- -5' |
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| 22062 | 5' | -57.4 | NC_004927.1 | + | 35029 | 1.11 | 0.000654 |
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Target: 5'- gCGUCACCUGAGCCAUAGCCGAGCGGCu -3' miRNA: 3'- -GCAGUGGACUCGGUAUCGGCUCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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