miRNA display CGI


Results 1 - 13 of 13 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22063 3' -51.1 NC_004927.1 + 18945 0.66 0.957878
Target:  5'- uUAGCUCCcucuCCGGGguu-CGAGGUcaugguguacGCCu -3'
miRNA:   3'- -GUCGAGGu---GGCUCaauuGCUUCA----------CGG- -5'
22063 3' -51.1 NC_004927.1 + 32903 0.66 0.95373
Target:  5'- cCAGCUCCAuCUGAGc-GGCGuuaucGAGUGaCCc -3'
miRNA:   3'- -GUCGAGGU-GGCUCaaUUGC-----UUCAC-GG- -5'
22063 3' -51.1 NC_004927.1 + 18892 0.66 0.949315
Target:  5'- aAGCUCCACCGuGUUGugcuUGAcAG-GCg -3'
miRNA:   3'- gUCGAGGUGGCuCAAUu---GCU-UCaCGg -5'
22063 3' -51.1 NC_004927.1 + 59664 0.66 0.944629
Target:  5'- gCGGCaagUCCAUCGAGauuGCGGAgcguGUGCUg -3'
miRNA:   3'- -GUCG---AGGUGGCUCaauUGCUU----CACGG- -5'
22063 3' -51.1 NC_004927.1 + 20320 0.66 0.939668
Target:  5'- gCGGCcCCACCgGAGggAGCGucGUcGUCg -3'
miRNA:   3'- -GUCGaGGUGG-CUCaaUUGCuuCA-CGG- -5'
22063 3' -51.1 NC_004927.1 + 8145 0.66 0.939668
Target:  5'- gGGCUUCAgUCGGGUUGuucCGAuGUGCUu -3'
miRNA:   3'- gUCGAGGU-GGCUCAAUu--GCUuCACGG- -5'
22063 3' -51.1 NC_004927.1 + 33172 0.67 0.923115
Target:  5'- --cCUCCGgCGGGUUGGCGGucgGCCc -3'
miRNA:   3'- gucGAGGUgGCUCAAUUGCUucaCGG- -5'
22063 3' -51.1 NC_004927.1 + 61375 0.69 0.864434
Target:  5'- -cGCUCCGCUGAGUgagggcguuuccguUAcCGAGGaUGCUc -3'
miRNA:   3'- guCGAGGUGGCUCA--------------AUuGCUUC-ACGG- -5'
22063 3' -51.1 NC_004927.1 + 14494 0.69 0.850223
Target:  5'- gUAGCUCaggauaGCCGAG--AACGAgAGUGUCu -3'
miRNA:   3'- -GUCGAGg-----UGGCUCaaUUGCU-UCACGG- -5'
22063 3' -51.1 NC_004927.1 + 73949 0.7 0.814228
Target:  5'- aCAGCU-CGCCGcAGUUGcgGCGGAG-GCUg -3'
miRNA:   3'- -GUCGAgGUGGC-UCAAU--UGCUUCaCGG- -5'
22063 3' -51.1 NC_004927.1 + 14244 0.7 0.793071
Target:  5'- -cGUUCCACCGGGgguuguccaugAACGucGUGUCg -3'
miRNA:   3'- guCGAGGUGGCUCaa---------UUGCuuCACGG- -5'
22063 3' -51.1 NC_004927.1 + 22219 0.73 0.642074
Target:  5'- cCAGCUCCucgaugacuuccuCCGGGUcgucAACGggGUcGCCg -3'
miRNA:   3'- -GUCGAGGu------------GGCUCAa---UUGCuuCA-CGG- -5'
22063 3' -51.1 NC_004927.1 + 35224 1.12 0.002523
Target:  5'- aCAGCUCCACCGAGUUAACGAAGUGCCc -3'
miRNA:   3'- -GUCGAGGUGGCUCAAUUGCUUCACGG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.