Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22065 | 5' | -51.9 | NC_004927.1 | + | 50056 | 0.66 | 0.942371 |
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Target: 5'- aGAA-GACUGUcCCUGAaaucaacgugGACGGUGGGg -3' miRNA: 3'- cCUUgCUGACGaGGACU----------UUGUCGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 41972 | 0.66 | 0.93732 |
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Target: 5'- cGAAUGGCUGUUCUccuccaAGCGGAa -3' miRNA: 3'- cCUUGCUGACGAGGacuuugUCGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 16356 | 0.66 | 0.93732 |
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Target: 5'- cGAacGCGACcGCUUcgagagggagCUGGAACgAGCGGAu -3' miRNA: 3'- cCU--UGCUGaCGAG----------GACUUUG-UCGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 7354 | 0.67 | 0.907941 |
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Target: 5'- cGGAACGGCuUGCUcgggucuugucCCUGAGuc-GUGGAc -3' miRNA: 3'- -CCUUGCUG-ACGA-----------GGACUUuguCGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 15365 | 0.67 | 0.901243 |
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Target: 5'- cGAGCGuuUGCUCCgaUGggGCAGUa-- -3' miRNA: 3'- cCUUGCugACGAGG--ACuuUGUCGccu -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 13307 | 0.68 | 0.85559 |
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Target: 5'- aGGAACGuCgGCUCC-GAAGuCAGCGa- -3' miRNA: 3'- -CCUUGCuGaCGAGGaCUUU-GUCGCcu -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 72086 | 0.71 | 0.687872 |
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Target: 5'- cGGGGCGACUuCgaCUUGAuGGCGGCGGAg -3' miRNA: 3'- -CCUUGCUGAcGa-GGACU-UUGUCGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 24658 | 0.72 | 0.66606 |
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Target: 5'- aGGACGACcGCUCCUugauaGGAACAggucGCGGGc -3' miRNA: 3'- cCUUGCUGaCGAGGA-----CUUUGU----CGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 50347 | 0.78 | 0.352299 |
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Target: 5'- cGAGCGGguCUGCaUCCgcugGAGGCGGCGGAg -3' miRNA: 3'- cCUUGCU--GACG-AGGa---CUUUGUCGCCU- -5' |
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| 22065 | 5' | -51.9 | NC_004927.1 | + | 36535 | 1.12 | 0.002189 |
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Target: 5'- uGGAACGACUGCUCCUGAAACAGCGGAg -3' miRNA: 3'- -CCUUGCUGACGAGGACUUUGUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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