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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22066 | 3' | -50 | NC_004927.1 | + | 29131 | 0.67 | 0.949064 |
Target: 5'- gUCGUagaagaagcACUUGCCGugaccguaguGACAGAgcgGGCCGg- -3' miRNA: 3'- -AGCA---------UGAACGGC----------UUGUCUa--UCGGUgu -5' |
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22066 | 3' | -50 | NC_004927.1 | + | 29939 | 0.68 | 0.922344 |
Target: 5'- uUCGUG-UUGCCGAACGG--GGCgCACc -3' miRNA: 3'- -AGCAUgAACGGCUUGUCuaUCG-GUGu -5' |
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22066 | 3' | -50 | NC_004927.1 | + | 36753 | 1.1 | 0.004048 |
Target: 5'- cUCGUACUUGCCGAACAGAUAGCCACAa -3' miRNA: 3'- -AGCAUGAACGGCUUGUCUAUCGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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