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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22068 | 3' | -59.1 | NC_004927.1 | + | 65185 | 0.67 | 0.550341 |
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Target: 5'- uCCACGCCUAcgGUCGCgucggucguguCUCUGACuCGg -3' miRNA: 3'- cGGUGUGGGUuaCAGCG-----------GGGGCUG-GC- -5' |
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| 22068 | 3' | -59.1 | NC_004927.1 | + | 897 | 0.69 | 0.448775 |
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Target: 5'- gGCCGgaaccggagagaacUACCCGGagacGUacuuaccCGCCCCCGACCGu -3' miRNA: 3'- -CGGU--------------GUGGGUUa---CA-------GCGGGGGCUGGC- -5' |
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| 22068 | 3' | -59.1 | NC_004927.1 | + | 5885 | 0.69 | 0.443223 |
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Target: 5'- -aCACACCaaaGGUGUCGCCgUCGuACUGg -3' miRNA: 3'- cgGUGUGGg--UUACAGCGGgGGC-UGGC- -5' |
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| 22068 | 3' | -59.1 | NC_004927.1 | + | 66813 | 0.74 | 0.216282 |
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Target: 5'- cCCGC-CCCAcggGUgGCCCCgCGACCGa -3' miRNA: 3'- cGGUGuGGGUua-CAgCGGGG-GCUGGC- -5' |
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| 22068 | 3' | -59.1 | NC_004927.1 | + | 37788 | 1.09 | 0.000679 |
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Target: 5'- uGCCACACCCAAUGUCGCCCCCGACCGu -3' miRNA: 3'- -CGGUGUGGGUUACAGCGGGGGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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