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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22068 | 5' | -55.5 | NC_004927.1 | + | 2339 | 0.75 | 0.283726 |
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Target: 5'- gGCGGUgUAGUCGGUGGCGagGaAGAACg -3' miRNA: 3'- gCGCCA-GUCAGUCGCCGUagC-UCUUG- -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 9765 | 0.67 | 0.72227 |
|
Target: 5'- -uCGGUCGGUCGGCguuguucacGGCGacUCGuGGGCu -3' miRNA: 3'- gcGCCAGUCAGUCG---------CCGU--AGCuCUUG- -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 12330 | 0.7 | 0.530826 |
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Target: 5'- -uUGGUCGGUCAGgucaGGCGUCGGGu-- -3' miRNA: 3'- gcGCCAGUCAGUCg---CCGUAGCUCuug -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 24191 | 0.67 | 0.72227 |
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Target: 5'- uCGCGGUacucggAGUCAGCGGgaaCGUCGAa--- -3' miRNA: 3'- -GCGCCAg-----UCAGUCGCC---GUAGCUcuug -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 29665 | 0.67 | 0.742895 |
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Target: 5'- aCGCGGcUUcGUCGGCGuagucuucGCuAUCGGGAACc -3' miRNA: 3'- -GCGCC-AGuCAGUCGC--------CG-UAGCUCUUG- -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 33847 | 0.66 | 0.792399 |
|
Target: 5'- aCG-GGUCGGUCGGCaucccguacucGGCggCGAGGu- -3' miRNA: 3'- -GCgCCAGUCAGUCG-----------CCGuaGCUCUug -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 37750 | 1.09 | 0.001366 |
|
Target: 5'- aCGCGGUCAGUCAGCGGCAUCGAGAACc -3' miRNA: 3'- -GCGCCAGUCAGUCGCCGUAGCUCUUG- -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 45438 | 0.67 | 0.753053 |
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Target: 5'- gGCGG-CGGuUCAGCucGGUAUCGuuAGCg -3' miRNA: 3'- gCGCCaGUC-AGUCG--CCGUAGCucUUG- -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 51802 | 0.74 | 0.357985 |
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Target: 5'- gGCGGUCGGUgAGCGuGCGacuccccaucugacUCGGGAAg -3' miRNA: 3'- gCGCCAGUCAgUCGC-CGU--------------AGCUCUUg -5' |
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| 22068 | 5' | -55.5 | NC_004927.1 | + | 65516 | 0.67 | 0.73263 |
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Target: 5'- gGCGGUUuugaccUCGGCGGCAaCGAaAGCg -3' miRNA: 3'- gCGCCAGuc----AGUCGCCGUaGCUcUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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