Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22069 | 3' | -57.4 | NC_004927.1 | + | 59358 | 0.66 | 0.726045 |
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Target: 5'- gGUCGG-CGCuCUCGGAgUgAGaCAGUa -3' miRNA: 3'- gCAGCCuGUG-GAGCCUgAgUCgGUCG- -5' |
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| 22069 | 3' | -57.4 | NC_004927.1 | + | 20079 | 0.66 | 0.705587 |
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Target: 5'- aCGUCGGAauCACCacguacaGGuCuUCGGUCAGCu -3' miRNA: 3'- -GCAGCCU--GUGGag-----CCuG-AGUCGGUCG- -5' |
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| 22069 | 3' | -57.4 | NC_004927.1 | + | 32804 | 0.66 | 0.695255 |
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Target: 5'- uCGUCGGGcCGCCguagcCGGugUagguguuGCCGGUg -3' miRNA: 3'- -GCAGCCU-GUGGa----GCCugAgu-----CGGUCG- -5' |
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| 22069 | 3' | -57.4 | NC_004927.1 | + | 36933 | 0.68 | 0.590405 |
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Target: 5'- --cCGGAgACUcagCGGGCUguucagccguaCAGCCAGCg -3' miRNA: 3'- gcaGCCUgUGGa--GCCUGA-----------GUCGGUCG- -5' |
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| 22069 | 3' | -57.4 | NC_004927.1 | + | 40217 | 0.7 | 0.4691 |
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Target: 5'- aCGaUUGGGaacccgACCUCGaACUCAGCCAGUc -3' miRNA: 3'- -GC-AGCCUg-----UGGAGCcUGAGUCGGUCG- -5' |
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| 22069 | 3' | -57.4 | NC_004927.1 | + | 38148 | 1.11 | 0.00076 |
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Target: 5'- uCGUCGGACACCUCGGACUCAGCCAGCu -3' miRNA: 3'- -GCAGCCUGUGGAGCCUGAGUCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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