Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22072 | 3' | -55.7 | NC_004927.1 | + | 51650 | 0.66 | 0.816309 |
Target: 5'- uCCAGUauaaCGACGGUGACGgcaacguugaacucuAuacugguaGCGGGACUg -3' miRNA: 3'- -GGUCAg---GUUGCCGCUGC---------------U--------CGCUCUGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 50800 | 0.66 | 0.812702 |
Target: 5'- -aAGUCauCAACGGCccgcCGGGUGGGGCUg -3' miRNA: 3'- ggUCAG--GUUGCCGcu--GCUCGCUCUGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 67818 | 0.66 | 0.784793 |
Target: 5'- aCCcGUCUAucucgcuccggGCGGCGGCGuGaCGcAGACUg -3' miRNA: 3'- -GGuCAGGU-----------UGCCGCUGCuC-GC-UCUGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 32904 | 0.66 | 0.775181 |
Target: 5'- uCCAGcUCCAucugaGCGGCGuuauCGAGUGAcccGCUg -3' miRNA: 3'- -GGUC-AGGU-----UGCCGCu---GCUCGCUc--UGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 64670 | 0.68 | 0.662627 |
Target: 5'- aCCGGcgugCUuACGGCG-CGAuGCGGGACa -3' miRNA: 3'- -GGUCa---GGuUGCCGCuGCU-CGCUCUGa -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 61337 | 0.69 | 0.635064 |
Target: 5'- cUCAGUCCAaggucaACGGaauGGCGAGCGAauacaaccuugcgcuGGCUa -3' miRNA: 3'- -GGUCAGGU------UGCCg--CUGCUCGCU---------------CUGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 55032 | 0.69 | 0.620205 |
Target: 5'- gCC-GUCCA-CGGUgcGACGuGUGAGGCUg -3' miRNA: 3'- -GGuCAGGUuGCCG--CUGCuCGCUCUGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 42327 | 0.74 | 0.358664 |
Target: 5'- aUCAGcCCGuugGCGGCGACGGGguaggcauCGAGGCUg -3' miRNA: 3'- -GGUCaGGU---UGCCGCUGCUC--------GCUCUGA- -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 54986 | 0.8 | 0.145466 |
Target: 5'- gUCAGUCC-ACGGUGACGGGaGAGACg -3' miRNA: 3'- -GGUCAGGuUGCCGCUGCUCgCUCUGa -5' |
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22072 | 3' | -55.7 | NC_004927.1 | + | 40190 | 1.1 | 0.001384 |
Target: 5'- gCCAGUCCAACGGCGACGAGCGAGACUa -3' miRNA: 3'- -GGUCAGGUUGCCGCUGCUCGCUCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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