miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22072 5' -55.8 NC_004927.1 + 34024 0.66 0.765595
Target:  5'- uGAGCCGUGUUCGccuucuuacacggguAGCCGgaGa---- -3'
miRNA:   3'- gCUCGGCGCAAGC---------------UCGGCgaCauagu -5'
22072 5' -55.8 NC_004927.1 + 33742 0.66 0.760624
Target:  5'- uCGAGCUGUcaaacucgauauaGUUCGAGUCGgUGUcgCc -3'
miRNA:   3'- -GCUCGGCG-------------CAAGCUCGGCgACAuaGu -5'
22072 5' -55.8 NC_004927.1 + 32892 0.66 0.751599
Target:  5'- uGAGCgGCGUuaUCGAGugacCCGCUGaccuUCGu -3'
miRNA:   3'- gCUCGgCGCA--AGCUC----GGCGACau--AGU- -5'
22072 5' -55.8 NC_004927.1 + 20106 0.71 0.479437
Target:  5'- -cAGCCGgGUUCGGGCCGUucucggUGUacGUCGg -3'
miRNA:   3'- gcUCGGCgCAAGCUCGGCG------ACA--UAGU- -5'
22072 5' -55.8 NC_004927.1 + 21801 0.73 0.394824
Target:  5'- gCGAGCCGC--UgGAGCCGCUGg---- -3'
miRNA:   3'- -GCUCGGCGcaAgCUCGGCGACauagu -5'
22072 5' -55.8 NC_004927.1 + 1134 0.76 0.263633
Target:  5'- aGGGCCG-GUUCGGGCCGCUu--UCAg -3'
miRNA:   3'- gCUCGGCgCAAGCUCGGCGAcauAGU- -5'
22072 5' -55.8 NC_004927.1 + 40156 1.09 0.001277
Target:  5'- aCGAGCCGCGUUCGAGCCGCUGUAUCAa -3'
miRNA:   3'- -GCUCGGCGCAAGCUCGGCGACAUAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.