Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22073 | 5' | -52.9 | NC_004927.1 | + | 38760 | 0.66 | 0.885278 |
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Target: 5'- gCUUCGUCGGCGucgaacuccgaguGCGCccaucCACGGGAAc -3' miRNA: 3'- gGGAGCAGUCGUu------------UGCGa----GUGCUCUU- -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 46601 | 0.67 | 0.856117 |
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Target: 5'- cCCCUgCGcCgAGCAGAUGCUCguggaguACGAGGc -3' miRNA: 3'- -GGGA-GCaG-UCGUUUGCGAG-------UGCUCUu -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 59381 | 0.67 | 0.839878 |
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Target: 5'- aCCCgagCGUCGGCGgcgguaauggucGGCGCUCucggagUGAGAc -3' miRNA: 3'- -GGGa--GCAGUCGU------------UUGCGAGu-----GCUCUu -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 39641 | 0.68 | 0.821938 |
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Target: 5'- cCUCUCGUgguaCAGCAucCGC-CACGGGGu -3' miRNA: 3'- -GGGAGCA----GUCGUuuGCGaGUGCUCUu -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 2176 | 0.7 | 0.678909 |
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Target: 5'- aCCUCGUgAGCuaa-GCUCACGGuGGAg -3' miRNA: 3'- gGGAGCAgUCGuuugCGAGUGCU-CUU- -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 22933 | 0.72 | 0.552218 |
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Target: 5'- uCCUUCGUCAGCuccucggaagcgucGAUGCUCacaACGAGAGc -3' miRNA: 3'- -GGGAGCAGUCGu-------------UUGCGAG---UGCUCUU- -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 28037 | 0.76 | 0.371117 |
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Target: 5'- uCCUUCGUCuccugagggAGUccGACGCUCACGAGAAc -3' miRNA: 3'- -GGGAGCAG---------UCGu-UUGCGAGUGCUCUU- -5' |
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| 22073 | 5' | -52.9 | NC_004927.1 | + | 40578 | 1.09 | 0.002644 |
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Target: 5'- cCCCUCGUCAGCAAACGCUCACGAGAAg -3' miRNA: 3'- -GGGAGCAGUCGUUUGCGAGUGCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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