Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22074 | 3' | -58.8 | NC_004927.1 | + | 49261 | 0.66 | 0.682275 |
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Target: 5'- -aGCGAGG-ACGcUCCCGACCaCGUu- -3' miRNA: 3'- ggUGCUCCuUGCaGGGGCUGG-GCGuc -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 4250 | 0.66 | 0.672023 |
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Target: 5'- uCCACGAGaGGCaGUCUCCGGuguCCCaCAGg -3' miRNA: 3'- -GGUGCUCcUUG-CAGGGGCU---GGGcGUC- -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 31509 | 0.66 | 0.65143 |
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Target: 5'- uCCG-GGGGAACGUagcaaCCCGACCU-CGGu -3' miRNA: 3'- -GGUgCUCCUUGCAg----GGGCUGGGcGUC- -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 27014 | 0.67 | 0.603938 |
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Target: 5'- cUCAuCGAGG-GCGUuacuagcuugagugcCCCCGACuuGUAGg -3' miRNA: 3'- -GGU-GCUCCuUGCA---------------GGGGCUGggCGUC- -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 2419 | 0.68 | 0.538746 |
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Target: 5'- uCCGCGAGGGGCuUCacaCCGAaUCCGCc- -3' miRNA: 3'- -GGUGCUCCUUGcAGg--GGCU-GGGCGuc -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 1440 | 0.72 | 0.341073 |
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Target: 5'- aCCACGGGGGcCGUCUCC--CCCGCu- -3' miRNA: 3'- -GGUGCUCCUuGCAGGGGcuGGGCGuc -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 61229 | 0.72 | 0.318484 |
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Target: 5'- cCCGCGuucAGGu-CGUCuaCCCGGCCCGCGa -3' miRNA: 3'- -GGUGC---UCCuuGCAG--GGGCUGGGCGUc -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 65485 | 0.73 | 0.283373 |
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Target: 5'- aCgGCGggagugguGGGAACGaCCCCGACCCGUc- -3' miRNA: 3'- -GgUGC--------UCCUUGCaGGGGCUGGGCGuc -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 49164 | 0.74 | 0.251396 |
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Target: 5'- aCUACGAGGAcgauuggagaGCGUCCCCuuCCgCGCAu -3' miRNA: 3'- -GGUGCUCCU----------UGCAGGGGcuGG-GCGUc -5' |
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| 22074 | 3' | -58.8 | NC_004927.1 | + | 42750 | 1.1 | 0.00071 |
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Target: 5'- uCCACGAGGAACGUCCCCGACCCGCAGg -3' miRNA: 3'- -GGUGCUCCUUGCAGGGGCUGGGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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