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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22077 | 3' | -51.2 | NC_004927.1 | + | 54276 | 0.68 | 0.916947 |
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Target: 5'- gCUCuUUCGACGgcgauacUGAGGAGgaaAUCCuCGa -3' miRNA: 3'- -GAGcAAGCUGCa------ACUCCUCa--UAGG-GC- -5' |
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| 22077 | 3' | -51.2 | NC_004927.1 | + | 13360 | 0.68 | 0.904137 |
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Target: 5'- cCUUGUUCGugauacagcuCGUaGA-GAGUAUCCCGg -3' miRNA: 3'- -GAGCAAGCu---------GCAaCUcCUCAUAGGGC- -5' |
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| 22077 | 3' | -51.2 | NC_004927.1 | + | 70078 | 0.68 | 0.890262 |
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Target: 5'- cCUCGcUgGACGaguUUGAcGGAGUGguUCCCGa -3' miRNA: 3'- -GAGCaAgCUGC---AACU-CCUCAU--AGGGC- -5' |
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| 22077 | 3' | -51.2 | NC_004927.1 | + | 69804 | 0.7 | 0.833877 |
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Target: 5'- aCUgGUguagagUCGGCGggUGGGGAGgucaaGUCCCGa -3' miRNA: 3'- -GAgCA------AGCUGCa-ACUCCUCa----UAGGGC- -5' |
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| 22077 | 3' | -51.2 | NC_004927.1 | + | 46650 | 1.11 | 0.003619 |
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Target: 5'- aCUCGUUCGACGUUGAGGAGUAUCCCGa -3' miRNA: 3'- -GAGCAAGCUGCAACUCCUCAUAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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