Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22078 | 3' | -56.1 | NC_004927.1 | + | 288 | 0.67 | 0.699971 |
|
Target: 5'- ---aGGggCAgggGCCGAcCGGCCCGAg -3' miRNA: 3'- gaggCCaaGUa--CGGCUuGUCGGGCUg -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 32043 | 0.73 | 0.352178 |
|
Target: 5'- gCUUCGGUUCGc-CCGAACAGUuuGGCu -3' miRNA: 3'- -GAGGCCAAGUacGGCUUGUCGggCUG- -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 33172 | 0.7 | 0.540483 |
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Target: 5'- cCUCCGGcggguUGgCGGuCGGCCCGGCg -3' miRNA: 3'- -GAGGCCaagu-ACgGCUuGUCGGGCUG- -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 47440 | 1.1 | 0.001041 |
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Target: 5'- gCUCCGGUUCAUGCCGAACAGCCCGACg -3' miRNA: 3'- -GAGGCCAAGUACGGCUUGUCGGGCUG- -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 53688 | 0.68 | 0.625337 |
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Target: 5'- ---gGGUUCGaGCgCGAcCGGCCCGGCu -3' miRNA: 3'- gaggCCAAGUaCG-GCUuGUCGGGCUG- -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 56477 | 0.69 | 0.593246 |
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Target: 5'- -cCCGGcg-AUGCuuaCGAACAGCUCGACa -3' miRNA: 3'- gaGGCCaagUACG---GCUUGUCGGGCUG- -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 60913 | 0.67 | 0.720883 |
|
Target: 5'- aUCCGGgUCAa--CGAACAGCCCu-- -3' miRNA: 3'- gAGGCCaAGUacgGCUUGUCGGGcug -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 71322 | 0.71 | 0.459836 |
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Target: 5'- cCUCCGGUUCAgGUgGAACAgacggaggaGCgCGACg -3' miRNA: 3'- -GAGGCCAAGUaCGgCUUGU---------CGgGCUG- -5' |
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| 22078 | 3' | -56.1 | NC_004927.1 | + | 75805 | 0.67 | 0.699971 |
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Target: 5'- ---aGGggCAgggGCCGAcCGGCCCGAg -3' miRNA: 3'- gaggCCaaGUa--CGGCUuGUCGGGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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