Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22079 | 3' | -48.6 | NC_004927.1 | + | 13675 | 0.66 | 0.986592 |
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Target: 5'- -uUGCCgUGAAuAUCGUAcAGCGCCgCCAUg -3' miRNA: 3'- cuAUGG-ACUU-UAGCGU-UUGUGG-GGUG- -5' |
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| 22079 | 3' | -48.6 | NC_004927.1 | + | 14777 | 0.66 | 0.984705 |
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Target: 5'- --gGCUUGccGUCGCGGagaagcacgacACGCUCCACg -3' miRNA: 3'- cuaUGGACuuUAGCGUU-----------UGUGGGGUG- -5' |
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| 22079 | 3' | -48.6 | NC_004927.1 | + | 34382 | 0.67 | 0.965423 |
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Target: 5'- ----gCUGAGGUCGUccaguuGCGCUCCACg -3' miRNA: 3'- cuaugGACUUUAGCGuu----UGUGGGGUG- -5' |
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| 22079 | 3' | -48.6 | NC_004927.1 | + | 18840 | 0.67 | 0.965423 |
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Target: 5'- aGUACCUcacccGUUGCGu-CACCCCACu -3' miRNA: 3'- cUAUGGAcuu--UAGCGUuuGUGGGGUG- -5' |
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| 22079 | 3' | -48.6 | NC_004927.1 | + | 67029 | 0.7 | 0.886921 |
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Target: 5'- --gACCUGAAG-CGCAA--GCUCCGCa -3' miRNA: 3'- cuaUGGACUUUaGCGUUugUGGGGUG- -5' |
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| 22079 | 3' | -48.6 | NC_004927.1 | + | 27973 | 0.78 | 0.510219 |
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Target: 5'- cGAUACUUGggGUC--AAACACCCCAUc -3' miRNA: 3'- -CUAUGGACuuUAGcgUUUGUGGGGUG- -5' |
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| 22079 | 3' | -48.6 | NC_004927.1 | + | 48077 | 1.09 | 0.006002 |
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Target: 5'- uGAUACCUGAAAUCGCAAACACCCCACc -3' miRNA: 3'- -CUAUGGACUUUAGCGUUUGUGGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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