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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22081 | 5' | -53.3 | NC_004927.1 | + | 56875 | 0.66 | 0.897816 |
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Target: 5'- aCGACCUCGauaaUCCGAuucuUGAGauucguccUCUCACg -3' miRNA: 3'- -GCUGGAGCaa--GGGCU----ACUC--------AGAGUGa -5' |
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| 22081 | 5' | -53.3 | NC_004927.1 | + | 71540 | 0.69 | 0.769454 |
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Target: 5'- aCGACCUCGUguggcuUCCCGAgGAuGaUUCGCUc -3' miRNA: 3'- -GCUGGAGCA------AGGGCUaCU-CaGAGUGA- -5' |
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| 22081 | 5' | -53.3 | NC_004927.1 | + | 25586 | 0.69 | 0.736974 |
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Target: 5'- aGACCUCGUUCCCGAacucguuuucgaGAacGUCUC-Cg -3' miRNA: 3'- gCUGGAGCAAGGGCUa-----------CU--CAGAGuGa -5' |
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| 22081 | 5' | -53.3 | NC_004927.1 | + | 14559 | 0.73 | 0.546691 |
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Target: 5'- -aGCCgaCGUUCCCGGUGGGUaguuaCUCACg -3' miRNA: 3'- gcUGGa-GCAAGGGCUACUCA-----GAGUGa -5' |
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| 22081 | 5' | -53.3 | NC_004927.1 | + | 54828 | 1.08 | 0.003044 |
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Target: 5'- aCGACCUCGUUCCCGAUGAGUCUCACUc -3' miRNA: 3'- -GCUGGAGCAAGGGCUACUCAGAGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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