Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22082 | 5' | -47 | NC_004927.1 | + | 11658 | 0.66 | 0.99777 |
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Target: 5'- -cGAGCCGACGAUGGUGuucguGUGAa- -3' miRNA: 3'- gcUUUGGCUGUUGCUACcuu--CACUac -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 67481 | 0.66 | 0.99777 |
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Target: 5'- uCGAAGCUGACcccGGCGAgUGGGAGaagcUGAg- -3' miRNA: 3'- -GCUUUGGCUG---UUGCU-ACCUUC----ACUac -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 52231 | 0.67 | 0.996177 |
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Target: 5'- uCGAccCgGACAACGAUGGGcAGgucgcgGAUGc -3' miRNA: 3'- -GCUuuGgCUGUUGCUACCU-UCa-----CUAC- -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 71744 | 0.69 | 0.981071 |
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Target: 5'- aGAAcCCGGCGGCGGUGGcuGAGcGAa- -3' miRNA: 3'- gCUUuGGCUGUUGCUACC--UUCaCUac -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 50043 | 0.7 | 0.973135 |
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Target: 5'- uGAAAUcaaCGugGACGGUGGGGuUGGUGg -3' miRNA: 3'- gCUUUG---GCugUUGCUACCUUcACUAC- -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 42855 | 0.7 | 0.963032 |
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Target: 5'- uCGAGGCCGACGAUG-UGGGgugucguacagaGGUcGGUGa -3' miRNA: 3'- -GCUUUGGCUGUUGCuACCU------------UCA-CUAC- -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 50244 | 0.72 | 0.929921 |
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Target: 5'- uGGAGgUGGCGGCGGUGGuGGUGGa- -3' miRNA: 3'- gCUUUgGCUGUUGCUACCuUCACUac -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 50394 | 0.74 | 0.858223 |
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Target: 5'- ---uGCCGGgAGCGGUGGuGGUGGUGc -3' miRNA: 3'- gcuuUGGCUgUUGCUACCuUCACUAC- -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 65494 | 0.74 | 0.840672 |
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Target: 5'- aCGAAAgCGACGGC---GGGAGUGGUGg -3' miRNA: 3'- -GCUUUgGCUGUUGcuaCCUUCACUAC- -5' |
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| 22082 | 5' | -47 | NC_004927.1 | + | 57362 | 1.11 | 0.008604 |
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Target: 5'- cCGAAACCGACAACGAUGGAAGUGAUGg -3' miRNA: 3'- -GCUUUGGCUGUUGCUACCUUCACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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